The potential involvement of Notch signaling in NK cell development

Autor: Rhodri Ceredig, Gina Balciunaite, Antonius G. Rolink, Corinne Demolière
Přispěvatelé: Developmental and Molecular Immunology, Department of Clinical and Biological Sciences (DKBW), University of Basel (Unibas), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Saas, Philippe
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Cellular differentiation
MESH: Flow Cytometry
MESH: Receptor
Notch1
MESH: Down-Regulation
Mice
Interleukin 21
0302 clinical medicine
Immunology and Allergy
MESH: Animals
Receptor
Notch1
0303 health sciences
Receptors
Notch
Janus kinase 3
Innate lymphoid cell
Intracellular Signaling Peptides and Proteins
Cell Differentiation
Flow Cytometry
MESH: Gene Expression Regulation
Cell biology
Killer Cells
Natural
MESH: NK Cell Lectin-Like Receptor Subfamily D
Interleukin 12
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
MESH: Membrane Proteins
NK Cell Lectin-Like Receptor Subfamily D
MESH: Killer Cells
Natural
MESH: Cell Differentiation
[SDV.IMM] Life Sciences [q-bio]/Immunology
Immunology
Notch signaling pathway
MESH: B-Cell-Specific Activator Protein
Down-Regulation
Thymus Gland
Biology
MESH: Coculture Techniques
03 medical and health sciences
MESH: Mice
Inbred C57BL
MESH: Intracellular Signaling Peptides and Proteins
Animals
Antigen-presenting cell
MESH: Mice
030304 developmental biology
Interleukin-7
Multipotent Stem Cells
PAX5 Transcription Factor
Membrane Proteins
MESH: Interleukin-2
MESH: Thymus Gland
Coculture Techniques
MESH: Interleukin-7
Mice
Inbred C57BL
Gene Expression Regulation
Myeloid-derived Suppressor Cell
Interleukin-2
MESH: Multipotent Stem Cells
MESH: Receptors
Notch
MESH: Female
030215 immunology
Zdroj: Immunology Letters
Immunology Letters, Elsevier, 2006, 107 (1), pp.50-7. ⟨10.1016/j.imlet.2006.07.005⟩
ISSN: 0165-2478
Popis: International audience; NK cells constitute an essential element of the innate immune system; however, the cellular and molecular mechanisms that guide their early development are still poorly understood. Here, we demonstrate that in addition to its known crucial role in T cell development, Notch signaling can also be involved in NK cell development. Thus, upon co-culture on OP9 stroma expressing the Notch ligand Delta-like 1 (OP9-DL1), Pax5-deficient pro-B cells, which have multi-lineage potential, efficiently differentiate into T and NK cells. Upon DL-1 signaling, Pax5-deficient pro-B cells down-regulate both surface CD93 expression and transcripts for B cell-specific genes and concomitantly up-regulate T lineage gene transcripts. Subsequent transfer of DL-1-signaled Pax5-deficient pro-B cells onto OP9 stroma in the presence of IL-2 leads to their efficient differentiation into NK1.1(+), functional NK cells. Moreover, bone marrow early progenitor with lymphoid and myeloid differentiation potential (EPLM), which we have previously described as the normal in vivo-equivalent of Pax5-deficient pro-B cells, also gain the ability to differentiate into effector NK cells following transient DL1 Notch-mediated signaling. The potential involvement of Notch signaling in the generation of the NK cell repertoire in vivo is discussed.
Databáze: OpenAIRE
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