Multidimensional Proteomic Analysis of the Soluble Subproteome of the Emerging Nosocomial Pathogen Ochrobactrum anthropi
| Autor: | Nigel G. Ternan, Geoffrey McMullan, Catherine E. Pollock, S. Naomi O'Loughlin, Rick L. Tarleton, Philip J. Jackson, D. Brent Weatherly, Robert Graham |
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| Rok vydání: | 2006 |
| Předmět: |
Proteomics
Ochrobactrum anthropi Proteome Molecular Sequence Data Virulence Peptide Computational biology Biochemistry Mass Spectrometry Microbiology Automation Bacterial Proteins Electrophoresis Gel Two-Dimensional Amino Acid Sequence Peptide sequence chemistry.chemical_classification biology General Chemistry biology.organism_classification Peptide Fragments Citric acid cycle chemistry Nucleic acid |
| Zdroj: | Journal of Proteome Research. 5:3145-3153 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | We report the first large-scale gel-free proteomic analysis of the soluble subproteome of the emerging pathogen Ochrobactrum anthropi. Utilizing our robust offline multidimensional protein identification protocol, a total of 57 280 peptides were initially identified utilizing automated MS/MS analysis software. We describe our investigation of the heuristic protein validation tool PROVALT and demonstrate its ability to increase the speed and accuracy of the curation process of large-scale proteomic datasets. PROVALT reduced our peptide list to 8517 identified peptides and further manual curation of these peptides led to a final list of 984 uniquely identified peptides that resulted in the positive identification of 249 proteins. These identified proteins were functionally classified and physiochemically characterized. A variety of typical "housekeeping" functions identified within the proteome included nucleic acid, amino and fatty acid anabolism and catabolism, glycolysis, TCA cycle, and pyruvate and selenoamino acid metabolism. In addition, a number of potential virulence factors of relevance to both plant and human disease were identified. |
| Databáze: | OpenAIRE |
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