Protective Effects of Flavonoids in Acute Models of Light-Induced Retinal Degeneration
| Autor: | Marcin Golczak, Joseph T. Ortega, Beata Jastrzebska, Tanu Parmar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Retinal degeneration Programmed cell death Mice 129 Strain genetic structures Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Electroretinography medicine Animals Receptor Flavonoids Mice Knockout Pharmacology Mice Inbred BALB C Retina biology Retinal Degeneration Retinal Articles medicine.disease Cell biology Mice Inbred C57BL Neuroprotective Agents 030104 developmental biology medicine.anatomical_structure chemistry Apoptosis Rhodopsin biology.protein Molecular Medicine Female Myricetin sense organs Photic Stimulation 030217 neurology & neurosurgery |
| Zdroj: | Mol Pharmacol |
| ISSN: | 1521-0111 0026-895X |
| Popis: | Degeneration of photoreceptors caused by excessive illumination, inherited mutations, or aging is the principal pathology of blinding diseases. Pharmacological compounds that stabilize the visual receptor rhodopsin and modulate the cellular pathways triggering death of photoreceptors could avert this pathology. Interestingly, flavonoids can modulate the cellular processes, such as oxidative stress, inflammatory responses, and apoptosis, that are activated during retinal degeneration. As we found previously, flavonoids also bind directly to unliganded rod opsin, enhancing its folding, stability, and regeneration. In addition, flavonoids stimulate rhodopsin gene expression. Thus, we evaluated the effect of two main dietary flavonoids, quercetin and myricetin, in ATP-binding cassette subfamily A member 4(−/−)/retinol dehydrogenase 8(−/−) and wild-type BALB/c mice susceptible to light-induced photoreceptor degeneration. Using in vivo imaging, such as optical coherence tomography, scanning laser ophthalmoscopy, and histologic assessment of retinal morphology, we found that treatment with these flavonoids prior to light insult remarkably protected retina from deterioration and preserved its function. Using high-performance liquid chromatography–mass spectrometry analysis, we detected these flavonoids in the eye upon their intraperitoneal administration. The molecular events associated with the protective effect of quercetin and myricetin were related to the elevated expression of photoreceptor-specific proteins, rhodopsin and cone opsins, decreased expression of the specific inflammatory markers, and the shift of the equilibrium between cell death regulators BCL2-associated X protein (BAX) and B-cell lymphoma 2 toward an antiapoptotic profile. These results were confirmed in photoreceptor-derived 661W cells treated with either H(2)O(2) or all-trans-retinal stressors implicated in the mechanism of retinal degeneration. Altogether, flavonoids could have significant prophylactic value for retinal degenerative diseases. SIGNIFICANCE STATEMENT: Flavonoids commonly present in food exhibit advantageous effects in blinding diseases. They bind to and stabilize unliganded rod opsin, which in excess accelerates degenerative processes in the retina. Additionally, flavonoids enhance the expression of the visual receptors, rod and cone opsins; inhibit the inflammatory reactions; and induce the expression of antiapoptotic markers in the retina, preventing the degeneration in vivo. Thus, flavonoids could have a prophylactic value for retinal degenerative diseases. |
| Databáze: | OpenAIRE |
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