Hydrogen sulfide protects from colitis and restores intestinal microbiota biofilm and mucus production
| Autor: | Jennifer K. Beatty, Kyle L. Flannigan, Gabriela Jorge Da Silva, Matthew L. Workentine, Andre G. Buret, Rory W. Blackler, Rui Wang, Terence A. Agbor, Jean-Paul Motta, John L. Wallace |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Hydrogen sulfide Inflammatory bowel disease Microbiology chemistry.chemical_compound Mice medicine Immunology and Allergy Animals Humans Microbiome Hydrogen Sulfide Colitis Intestinal Mucosa Rats Wistar Barrier function In Situ Hybridization Fluorescence Gastrointestinal tract Chemistry Gasotransmitters Dextran Sulfate Gastroenterology Biofilm equipment and supplies medicine.disease Gastrointestinal Microbiome Rats Mice Inbred C57BL Mucus Biofilms Dysbiosis |
| Zdroj: | Inflammatory bowel diseases. 21(5) |
| ISSN: | 1536-4844 |
| Popis: | Microbiota dysbiosis and impaired barrier function are among the most prominent features of inflammatory bowel disease. In the gastrointestinal tract, hydrogen sulfide (H(2)S) is an important regulator of mucosal homeostasis. We hypothesized that H(2)S promotes resolution of colonic inflammation through actions on microbiota biofilm and the mucus barrier.We used mice genetically deficient for a key enzyme for H(2)S production (cystathionine γ-lyase) and pharmacologically inhibited that enzyme during colitis in wild-type mice. We tested the effects of administering an H(2)S donor (diallyl disulfide) to rodents during hapten-induced colitis. Colonic microbiota biofilm was visualized by fluorescent in situ hybridization, and mucus granules were quantified with periodic acid-alcian blue staining. We exposed human microbiota biofilms and planktonic bacteria to H(2)S donors ex vivo to determine changes in their growth, viability, and biomass.Intestinal microbiota formed linear biofilms in the colon of healthy rodents. During colitis, microbiota biofilms were fragmented and mucus granule production decreased. Endogenous production of H(2)S had beneficial effects on establishment of microbiota biofilms and colonic mucus production. Therapeutic delivery of H(2)S into the colon reduced inflammation, restored the microbiota biofilm, and increased the production of mucus granules. In ex vivo human microbiota, H(2)S not only promoted biofilm formation but also reduced growth of planktonic bacteria.Our results suggest that H(2)S donors could be used therapeutically during colitis, facilitating correction of microbiota biofilm dysbiosis and mucus layer reconstitution. |
| Databáze: | OpenAIRE |
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