Formation of acetoacetate from 3-hydroxy-3-methylglutarate by rat liver and isolation of a mitochondrial coenzyme A-transferase activity involved
| Autor: | R. Deana, L. Manzi, R. Meneghello, C. Gregolin |
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| Rok vydání: | 1974 |
| Předmět: |
Male
Stereochemistry Coenzyme A Centrifugation Mitochondria Liver Biology In Vitro Techniques Biochemistry 3-Methylglutarate Acetoacetates Glutarates chemistry.chemical_compound Transferase Animals Carbon Radioisotopes Molecular Biology Biosynthesis and Degradation Succinates Cell Biology Rats Perfusion Kinetics Freeze Drying chemistry Liver Rat liver Sulfurtransferases Subcellular Fractions |
| Zdroj: | The Biochemical journal. 138(3) |
| ISSN: | 0264-6021 |
| Popis: | 1. Formation of acetoacetate from 3-hydroxy-3-methylglutarate was observed in the perfused rat liver. Production of 3.5μmol of acetoacetate/h per g of tissue was obtained. 2. Formation of acetoacetate was catalysed mainly by the mitochondrial fraction of the homogenized liver, at a rate of 62nmol/h per mg of protein. 3. Experiments with hydroxy-[3-14C]methylglutarate demonstrated that the acetoacetate formed was derived mainly from this compound. 4. A mitochondrial transferase activity catalysing the transfer of a CoA molecule from succinyl-CoA (3-carboxypropionyl-CoA) to hydroxymethylglutarate was shown. The Km value for hydroxymethylglutarate was 5×10−3m. |
| Databáze: | OpenAIRE |
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