Posttranscriptional gene silencing of gn1 in tobacco triggers accumulation of truncated gn1-derived RNA species
| Autor: | John J. M. R. Jacobs, Gerben J. van Eldik, Katia Litière, Marc Van Montagu, Marc Cornelissen |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Messenger RNA
Polyadenylation Base Sequence DNA Plant beta-Glucosidase Trans-acting siRNA Molecular Sequence Data RNA Glucan 1 3-beta-Glucosidase Biology Genes Plant Plants Genetically Modified Molecular biology RNA silencing Plants Toxic Rapid amplification of cDNA ends RNA Plant Protein Biosynthesis Tobacco Gene silencing Gene Silencing Molecular Biology Gene Research Article |
| Popis: | Posttranscriptional silencing of basic beta-1,3-glucanase genes in the tobacco line T17 is manifested by reduced transcript levels of the gn1 transgene and homologous, endogenous basic beta-1,3-glucanase genes. An RNA ligation-mediated rapid amplification of cDNA ends (RLM-RACE) technique was used to compare the 3' termini of gn1 RNAs present in expressing (hemizygous and young homozygous) and silenced (mature homozygous) T17 plants. Full-length, polyadenylated gn1 transcripts primarily accumulated in expressing plants, whereas in silenced T17 plants, mainly 3'-truncated, nonpolyadenylated gn1 RNAs were detected. The relative abundance of these 3'-truncated gn1 RNA species gradually increased during the establishment of silencing in homozygous T17 plants. Similar 3'-truncated, nonpolyadenylated gn1 RNA products were observed in an independent case of beta-1,3-glucanase posttranscriptional gene silencing. This suggests that these 3'-truncated gn1 RNAs are a general feature of tobacco plants showing posttranscriptional silencing of the gn1 transgene. |
| Databáze: | OpenAIRE |
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