Comparative Leaves Transcriptome Analysis Emphasizing on Accumulation of Anthocyanins in Brassica: Molecular Regulation and Potential Interaction with Photosynthesis
Autor: | Daozong Chen, Xianhong Ge, Qi Pan, Zaiyun Li, Qinghua Zhang, Muhammad Adnan Mushtaq |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Photosystem II medicine.medical_treatment Brassica Plant Science lcsh:Plant culture Biology Photosynthesis 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Glutamine synthetase Botany medicine lcsh:SB1-1110 pigmentation Gene Original Research Protease photosynthesis fungi food and beverages biology.organism_classification anthocyanins 030104 developmental biology chemistry Biochemistry Anthocyanin transcriptome 010606 plant biology & botany |
Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 7 (2016) |
ISSN: | 1664-462X |
Popis: | The purple leaf pigmentation mainly associated with anthocyanins accumulation is common in Brassica but the mechanisms of its production and its potential physiological functions are poorly understood. Here, we performed the phenotypic, cytological, physiological and comparative leaves transcriptome analyses of 11 different varieties belonging to five Brassica species with purple or green leaves. We observed that the anthocyanin was accumulated in most of vegetative tissues in all Brassica species and also in reproduction organs of B. carinata. Anthocyanin accumulated in different part of purple leaves including adaxial and abaxial epidermal cells as well as palisade and spongy mesophyll cells. Leave transcriptome analysis showed that almost all late biosynthetic genes of anthocyanin especially Dihydroflavonol 4-Reductase (DFR), Anthocyanidin Synthase (ANS) and Transparent Testa 19 (TT19), were highly up-regulated in all purple leaves. However, only one of transcript factors in anthocyanin biosynthesis pathway, Transparent Testa 8 (TT8), was co-up regulated along with those genes in all purple leaves, indicating its pivotal role for anthocyanin production in Brassica. Interestingly, with the up-regulation of genes for anthocyanin synthesis, Cytosolic 6-phosphogluconolactonase (PLG5) which involved in the oxidative pentose-phosphate pathway was up-regulated in all purple leaves and three genes FTSH PROTEASE 8 (FTS8), GLYCOLATE OXIDASE 1 (GOX1) and GLUTAMINE SYNTHETASE 1;4 (GLN1;4) related to degradation of photo-damaged proteins in photosystem II and light respiration were down-regulated. These results highlighted the potential physiological functions of anthocyanin accumulation related to photosynthesis which might be of great worth in future. |
Databáze: | OpenAIRE |
Externí odkaz: |