Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
| Autor: | Claudia Albrecht, Sabina I. Ramos Elizagaray, Elio Andres Soria, Guillermina A. Bongiovanni, Juan Jose Cantero, Patricia L. Quiroga |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Farmacología y Farmacia
Antioxidant CIENCIAS MÉDICAS Y DE LA SALUD Article Subject medicine.medical_treatment chemistry.chemical_element Biology Toxicology chemistry.chemical_compound Chlorogenic acid ANTIOXIDANTS medicine Pharmacology (medical) Food science General Pharmacology Toxicology and Pharmaceutics Nitrite Arsenic LANTANA GRISEBACHII chemistry.chemical_classification Arsenic toxicity LYMPHOPROTECTIVE ACTIVITY Verbenaceae lcsh:RM1-950 ARSENIC TOXICITY Fatty acid purl.org/becyt/ford/3.1 [https] biology.organism_classification Medicina Básica lcsh:Therapeutics. Pharmacology chemistry Toxicity Molecular Medicine purl.org/becyt/ford/3 [https] Research Article |
| Zdroj: | Advances in Pharmacological Sciences, Vol 2014 (2014) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Advances in Pharmacological Sciences |
| Popis: | Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae)(LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T- Activated cell cultures (P < 0.0001). Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG- 1i at 100g/mL), which protected them against in vitro As-induced lymphotoxicity (P < 0.0001). This infusive LG phytoextract contained 10.23 ± 0.43mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of 9, followed by 6 (∼24%) and 3 (∼4%), and scarce 7. Summing up, L. grisebachiiwas a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions Fil: Soria, Elio Andres. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Quiroga, Patricia L.. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Ramos Elizagaray, Sabina I.. Consejo Interuniversitario Nacional; Argentina Fil: Cantero, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Bongiovanni, Guillermina Azucena. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina |
| Databáze: | OpenAIRE |
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