Internalization of Vectorized Liposomes Loaded with Plasmid DNA in C6 Glioma Cells
| Autor: | F. A. Koshkin, Vladimir P. Chekhonin, A. N. Gabashvili, S. A. Cherepanov, P. A. Mel’nikov, A. V. Leopol’d, N. V. Nukolova, I. I. Kuznetsov, Vladimir P. Baklaushev |
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| Rok vydání: | 2017 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine medicine.drug_class Genetic enhancement media_common.quotation_subject Monoclonal antibody General Biochemistry Genetics and Molecular Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Glioma medicine Animals Internalization media_common Liposome Microscopy Confocal medicine.diagnostic_test Brain Neoplasms Chemistry Genetic Therapy General Medicine Transfection Flow Cytometry medicine.disease Vascular Endothelial Growth Factor Receptor-2 Molecular biology Rats 030104 developmental biology Liposomes Nanocarriers 030217 neurology & neurosurgery Plasmids |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 163:114-122 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-017-3750-x |
| Popis: | We studied internalization of vector nanocarriers loaded with plasmid DNA into C6 glioma cells. For improving selectivity of plasmid delivery, the liposomes were conjugated with monoclonal antibodies to VEGF and its receptor VEGFR2. Flow cytofluorometry and laser scanning confocal microscopy showed more intensive (more than 2-fold) internalization and accumulation of antibody-vectorized liposomes in C6 glioma cells in comparison with the control (liposomes conjugated with non-specific antibodies and non-vectorized liposomes). Using quantitative analysis of fluorescent signal, we showed that cationic immunoliposomes significantly more effective delivered pCop-Green-N plasmid DNA and ensured effective transfection of C6 glioma cells. |
| Databáze: | OpenAIRE |
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