Evaluation of various preservation media for storage of donor corneas
| Autor: | Tushar Agarwal, Jeewan S Titiyal, Namrata Sharma, Farin Shaikh, Ritu Nagpal, Rajesh Sinha, Prafulla K. Maharana, Thirumurthy Velpandian, Gita Satpathy, Manumuraleekrishna |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Distance visual acuity Special Focus Ocular Surface and Cornea Original Article Corneal preservation MK medium Cornisol medium Endothelial cell count Culture Media Serum-Free Cornea Ophthalmology Optisol-GS medium Medicine Humans business.industry Significant difference Endothelium Corneal Organ Preservation RE1-994 Normal limit Tissue Donors Endothelial cell density Endothelial stem cell Full thickness sense organs business donor corneal storage |
| Zdroj: | Indian Journal of Ophthalmology Indian Journal of Ophthalmology, Vol 69, Iss 9, Pp 2452-2456 (2021) |
| ISSN: | 1998-3689 |
| Popis: | Purpose: To compare the physical and microbiological characteristics of McCarey-Kaufman (MK), Cornisol, and Optisol-GS media and evaluate the outcomes of keratoplasty performed using corneas stored in these three media. Methods: The study involved 60 donor corneas which were distributed in 3 groups: MK, Cornisol, and Optisol-GS. Corneas in these groups were further analyzed based on the type of keratoplasty performed (full thickness versus endothelial keratoplasty). At baseline, the endothelial cell density and death to preservation time of donor corneas were recorded. Following keratoplasty, patients were evaluated on day 1, at 1 month, 3 months, and 6 months follow-up. Outcomes were assessed in terms of corrected distance visual acuity (CDVA), endothelial cell density, percentage endothelial cell loss, and corneal thickness. The storage media were also assessed for their physical quality and their microbiological characteristics. Results: Physical characteristics of all three media were found to be within normal limits. Mean CDVA was comparable among the 3 groups at 6-month follow-up. The absolute endothelial cell count values were significantly lower for corneas stored in MK medium (1873.7 ± 261.1 cells/mm2) compared to the Cornisol (2085.0 ± 230.3 cells/mm2) and Optisol-GS media [(2180.3 ± 217.2 cells/mm2) (P = |
| Databáze: | OpenAIRE |
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