Improved endothelialization and reduced thrombosis by coating a synthetic vascular graft with fibronectin and stem cell homing factor SDF-1α
| Autor: | Lindsay Mesure, Anna Ivanova, G. De Visscher, Willem Flameng, Bart Meuris |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Intimal hyperplasia Biomedical Engineering CD34 Biochemistry Biomaterials Coated Materials Biocompatible Blood vessel prosthesis Materials Testing Cell Adhesion medicine Animals Molecular Biology Sheep biology CD117 Stem Cells Endothelial Cells Thrombosis General Medicine medicine.disease Chemokine CXCL12 Blood Vessel Prosthesis Fibronectins Surgery Fibronectin Carotid Arteries surgical procedures operative biology.protein Female Stem cell Biotechnology Homing (hematopoietic) |
| Zdroj: | Acta Biomaterialia. 8:1330-1338 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2011.09.016 |
| Popis: | Failure of synthetic small-diameter vascular grafts is determined mainly by the lack of endothelial cells, as these cells inhibit thrombosis and intimal hyperplasia. Coating of graft material with homing factors for circulating stem cells has the potential to improve endogenous endothelialization of these grafts and to reduce graft failure. Synthetic knitted polyester grafts (6mm diameter) were coated with FN and SDF-1α before surgical interposition in the carotid artery of sheep. Similar uncoated vascular grafts were implanted in the contralateral side as internal controls. To study the early attraction of stem cells, grafts were implanted in a first series of nine sheep and explanted after 1 or 3 days. In coated grafts, four times higher fractions of CD34(+) and three to four times higher fractions of CD117(+) cells adhering to the vessel walls were found than in control grafts (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect