Screening of complex fucoidans from four brown algae species as procoagulant agents
| Autor: | Catherine Quinn, Sabine Knappe, Zhenqing Zhang, Christina M. Szabo, Friedrich Scheiflinger, James Catarello, Susanne Till, G. Joseph Ray, Michael Dockal |
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| Rok vydání: | 2015 |
| Předmět: |
Polymers and Plastics
Alginates Lipoproteins Phaeophyta Polysaccharide Glycosaminoglycan chemistry.chemical_compound Tissue factor pathway inhibitor Sulfation Polysaccharides Materials Chemistry Humans Blood Coagulation chemistry.chemical_classification biology Coagulants Fucoidan Monosaccharides Organic Chemistry Anticoagulants biology.organism_classification Brown algae chemistry Coagulation Biochemistry Hemostasis Partial Thromboplastin Time |
| Zdroj: | Carbohydrate Polymers. 115:677-685 |
| ISSN: | 0144-8617 |
| Popis: | Fucoidans are complex sulfated polysaccharides extracted from brown algae. Depending on the concentration, they have been shown to stimulate and inhibit blood coagulation in vitro. Promotion of coagulation is mediated by blocking tissue factor pathway inhibitor (TFPI). We screened fucoidan extracts from four brown algae species in vitro with respect to their potential to improve coagulation in bleeding disorders. The fucoidans' pro- and anticoagulant activities were assessed by global hemostatic and standard clotting assays. Results showed that fucoidans improved coagulation parameters. Some fucoidans also activated the contact pathway of coagulation, an undesired property reported for sulfated glycosaminoglycans. Chemical evaluation of fucoidans' complex and variable structure included molecular weight (Mw), polydispersity (polyD), structural heterogeneity, and organic and inorganic impurities. Herewith, we describe a screening strategy that facilitates the identification of crude fucoidan extracts with desired biological and structural properties for improvement of compromised coagulation like in hemophilia. |
| Databáze: | OpenAIRE |
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