Expression of survivin and Bcl-2 in the normal human endometrium
| Autor: | Ryo Konno, Akira Yajima, Hiroki Utsunomiya, S. Sato, Kiyoshi Ito, Hiromitsu Yamakawa |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Embryology medicine.medical_specialty Survivin Biology Endometrium Inhibitor of apoptosis Inhibitor of Apoptosis Proteins Reference Values Internal medicine Gene expression Follicular phase Genetics medicine Humans neoplasms Molecular Biology Menstrual Cycle Reverse Transcriptase Polymerase Chain Reaction Proteins Obstetrics and Gynecology Cell Biology Middle Aged Immunohistochemistry Neoplasm Proteins medicine.anatomical_structure Endocrinology Real-time polymerase chain reaction Proto-Oncogene Proteins c-bcl-2 Reproductive Medicine Apoptosis Cancer research Female Microtubule-Associated Proteins Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 6:529-534 |
| ISSN: | 1460-2407 |
| Popis: | Survivin is a novel inhibitor of apoptosis. It has been reported that survivin is expressed during fetal development and in cancer tissues, but its expression has not been reported in adult tissues. We investigated the expression of survivin in the endometria of women with regular menstrual cycles using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, and compared these findings with Bcl-2, an apoptosis inhibitor. Survivin mRNA was detected by RT-PCR in all samples (nine of nine) of endometrium during the secretory phase, but in only four out of seven samples from endometrium during the proliferative phase, and in none of the atrophic endometrium. Immunohistochemistry demonstrated a survivin protein expression that was strongest in the nuclei of glandular epithelial cells during the late secretory phase. In the proliferative phase, glandular epithelial cells were not stained for survivin. The cyclic changes of survivin and Bcl-2 showed an inverse relationship, with Bcl-2 expression being strongest in the proliferative phase and survivin expression being strongest in the secretory phase. The up-regulation of survivin expression may be due to the concurrent rise in progesterone concentrations during the normal menstrual cycle. Moreover, survivin could play an important role independent of Bcl-2 in physiological homeostasis in the normal endometrium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|