Laboratory Maintenance and Propagation of Freshwater Planarians
| Autor: | Elizabeth M. Duncan, Makayla R. P. Dean |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
ved/biology.organism_classification_rank.species
Fresh Water Microbiology Article Girardia 03 medical and health sciences Schmidtea mediterranea Virology Animals Model organism 030304 developmental biology 0303 health sciences biology Clinical Laboratory Techniques 030306 microbiology ved/biology Regeneration (biology) Cell Differentiation Planarians biology.organism_classification Animal Feed Cell biology Salt solution Liver Planarian Salts Parasitology Food preparation |
| Zdroj: | Curr Protoc Microbiol |
| ISSN: | 1934-8533 1934-8525 |
| DOI: | 10.1002/cpmc.120 |
| Popis: | Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians are easy to maintain, inexpensive to propagate, and reasonably macroscopic (1 mm to 1 cm in length), making them excellent organisms to use in both complex academic research and hands-on teaching laboratories. Here, we provide a detailed description of how to maintain and propagate these incredibly versatile animals in any basic laboratory setting. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Salt solution preparation Basic Protocol 2: Cleaning planarian housing Basic Protocol 3: Food preparation Basic Protocol 4: Feeding planarians Basic Protocol 5: Expansion and amplification of colony. |
| Databáze: | OpenAIRE |
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