Extracellular targeting of the vacuolar tobacco proteins AP24, chitinase and beta-1,3-glucanase in transgenic plants
Autor: | Van Roekel Js, Leo Sjoerd Melchers, Ben J. C. Cornelissen, Vloemans Sa, van den Elzen Pj, Pen J, Woloshuk Cp, Sela-Buurlage Mb |
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Rok vydání: | 1993 |
Předmět: |
Signal peptide
Phytophthora Nicotiana tabacum Recombinant Fusion Proteins Mutant Molecular Sequence Data DNA Single-Stranded Plant Science Chimeric gene Vacuole Genes Plant Mutant protein Tobacco Genetics Tobacco mosaic virus Amino Acid Sequence Cloning Molecular Protein Precursors Peptide sequence Plant Proteins biology Base Sequence beta-Glucosidase Chitinases Biological Transport General Medicine Glucan 1 3-beta-Glucosidase biology.organism_classification Plants Genetically Modified Cell Compartmentation Plants Toxic Biochemistry Vacuoles Agronomy and Crop Science Protein Processing Post-Translational |
Zdroj: | Plant molecular biology. 21(4) |
ISSN: | 0167-4412 |
Popis: | The Nicotiana tabacum ap24 gene encoding a protein with antifungal activity toward Phytophthora infestans has been characterized. Analysis of cDNA clones revealed that at least three ap24-like genes are induced in tobacco upon infection with tobacco mosaic virus. Amino acid sequencing of the purified protein showed that AP24 is synthesized as a preproprotein from which an amino-terminal signal peptide and a carboxyl-terminal propeptide (CTPP) are cleaved off during post-translational processing. The functional role of the CTPP was investigated by expressing chimeric genes encoding either wild-type AP24 or a mutant protein lacking the CTPP. Plants expressing the wild-type construct resulted in proteins properly sorted to the vacuole. In contrast, the proteins produced in plants expressing the mutant construct were secreted extracellularly, indicating that the CTPP is necessary for targeting of AP24 to the vacuoles. Similar results were obtained for vacuolar chitinases and beta-1,3-glucanases of tobacco. The extracellularly targeted mutant proteins were shown to have retained their biological activity. Together, these results suggest that within all vacuolar pathogenesis-related proteins the targeting information resides in a short carboxyl-terminal propeptide which is removed during or after transport to the plant vacuole. |
Databáze: | OpenAIRE |
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