Pathogen receptor discovery with a microfluidic human membrane protein array
| Autor: | Gregory Neveu, Michal Pellach, Yair Glick, Ariella Oppenheim, Ya'ara Ben-Ari, Jim Boonyaratanakornkit, Nir Drayman, Doron Gerber, Dorit Avrahami, Shirit Einav |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteomics Multidisciplinary 030102 biochemistry & molecular biology Cell Protein Array Analysis Simian virus 40 Biology Microfluidic Analytical Techniques Biological Sciences Virus Cell biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Membrane protein Viral envelope medicine Humans Receptors Virus Receptor Pathogen Tropism |
| Zdroj: | Proceedings of the National Academy of Sciences |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1518698113 |
| Popis: | The discovery of how a pathogen invades a cell requires one to determine which host cell receptors are exploited. This determination is a challenging problem because the receptor is invariably a membrane protein, which represents an Achilles heel in proteomics. We have developed a universal platform for high-throughput expression and interaction studies of membrane proteins by creating a microfluidic-based comprehensive human membrane protein array (MPA). The MPA is, to our knowledge, the first of its kind and offers a powerful alternative to conventional proteomics by enabling the simultaneous study of 2,100 membrane proteins. We characterized direct interactions of a whole nonenveloped virus (simian virus 40), as well as those of the hepatitis delta enveloped virus large form antigen, with candidate host receptors expressed on the MPA. Selected newly discovered membrane protein–pathogen interactions were validated by conventional methods, demonstrating that the MPA is an important tool for cellular receptor discovery and for understanding pathogen tropism. |
| Databáze: | OpenAIRE |
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