Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening
| Autor: | Marta Farková, Simona Macuchová, Blanka Hégrová, Jan Preisler, Josef Havel |
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| Rok vydání: | 2009 |
| Předmět: |
Fusarium
Antifungal Agents Filtration and Separation Peptide Fatty Acid-Binding Proteins Plant Proteins Dietary 01 natural sciences Analytical Chemistry 03 medical and health sciences Polyacrylamide gel electrophoresis Defensin Heat-Shock Proteins Czech Republic Plant Proteins 030304 developmental biology Gel electrophoresis chemistry.chemical_classification 0303 health sciences Chromatography biology Chemistry 010401 analytical chemistry Fungi Beer food and beverages Hordeum Fungi imperfecti biology.organism_classification 0104 chemical sciences Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome Electrophoresis Polyacrylamide Gel Carrier Proteins Plant lipid transfer proteins Antimicrobial Cationic Peptides |
| Zdroj: | Journal of Separation Science. 32:4247-4253 |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.200900510 |
| Popis: | The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (/r/ in0.07, 0.34), (/r/ in0.01, 0.49), respectively, as detected by both methods. |
| Databáze: | OpenAIRE |
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