Carbon nanotube-lipase hybrid nanoflowers with enhanced enzyme activity and enantioselectivity
| Autor: | Miaad Adnan Abdulrazaq, Jianhua Wang, Yaojia He, Yunjun Yan, Kai Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Vinyl Compounds
Immobilized enzyme Bioengineering 02 engineering and technology Carbon nanotube 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Phosphates law.invention chemistry.chemical_compound law Vinyl acetate Lipase Enantiomeric excess Benzyl Alcohols biology Chemistry Substrate (chemistry) Stereoisomerism General Medicine Enzymes Immobilized 021001 nanoscience & nanotechnology Lauric acid Enzyme assay Nanostructures 0104 chemical sciences Chemical engineering Microscopy Electron Scanning biology.protein 0210 nano-technology Biotechnology |
| Zdroj: | Journal of Biotechnology. 281:87-98 |
| ISSN: | 0168-1656 |
| Popis: | Various nanoflowers are synthesized as supports for different methods of enzyme immobilization; however, the activities of these immobilized enzymes are limited because of their confinement in the nanoflowers. In order to increase the performance of nanoflowers, in this study, different protein-phosphate hybrid nanostructures were successfully synthesized and further enhanced by carbon nanotubes (CNTs) under the same conditions. Only Cu3(PO4)2 complex nanostructures exhibited flower-like structures and showed excellent results after enhancement with CNTs in this framework. An esterification reaction between lauric acid and 1-dodecanol was used to test enzyme activity during immobilization, revealing that the Cu3(PO4)2/CNT/protein complex exhibited 68-fold higher activity relative to free lipase and 51-fold higher than that of Cu3(PO4)2/Burkholderia cepacia lipase hybrid nanoflowers in the absence of CNTs. All three hybrid nanostructures showed good performance and exhibited excellent reusability in resolution reactions between 1-phenylethanol and vinyl acetate. Additionally, the substrate enantiomeric excess (ees) reached 98% in only 10 min, and the corresponding Cu3(PO4)2/CNT/protein complex could be recycled eight times without obvious loss of activity. This approach involving nanoflowers enhanced with CNTs will be highly beneficial for decreasing mass-transfer resistance and providing enhanced enzyme loading along with promising potential for industrial application. |
| Databáze: | OpenAIRE |
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