In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics
| Autor: | Robin Siadous, Jean-Christophe Fricain, Raphaël Devillard, Jérôme Kalisky, Reine Bareille, Olivier Chassande, Murielle Rémy, J. Amédée-Vilamitjana, Jean-Michel Bourget, Olivia Kérourédan |
|---|---|
| Přispěvatelé: | Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux], CHU Bordeaux [Bordeaux], Chassande, Olivier |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Scaffold
Regenerative endodontics Alginates [SDV]Life Sciences [q-bio] regenerative medicine 02 engineering and technology adult stem cells Regenerative medicine Extracellular matrix 03 medical and health sciences 0302 clinical medicine Glucuronic Acid Tissue engineering Humans Regeneration General Dentistry Osteoblasts Tissue Scaffolds Chemistry tissue engineering Hexuronic Acids Mesenchymal stem cell Mesenchymal Stem Cells 030206 dentistry 021001 nanoscience & nanotechnology [SDV] Life Sciences [q-bio] endodontics cell differentiation Collagen Gutta-Percha Stem cell hydrogel 0210 nano-technology Adult stem cell Biomedical engineering |
| Zdroj: | International Endodontic Journal International Endodontic Journal, Wiley, 2017, 50 (1), pp.48-57. ⟨10.1111/iej.12591⟩ Europe PubMed Central |
| ISSN: | 0143-2885 1365-2591 |
| Popis: | International audience; Aim: To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.Methodology: A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t-tests and the Mann-Whitney U test were performed.Results: The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).Conclusions: The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text