Acidic pH Triggers Lipid Mixing Mediated by Lassa Virus GP
| Autor: | James B. Munro, Uriel Bulow, Ramesh Govindan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Endosome 030106 microbiology membrane fusion lcsh:QR1-502 Endosomes virus entry medicine.disease_cause Article lcsh:Microbiology 03 medical and health sciences Viral envelope Viral Envelope Proteins Viral entry single-particle fusion Virology Chlorocebus aethiops medicine Animals Humans Lassa virus Vero Cells Late endosome LAMP1 Chemistry Lipid bilayer fusion Lysosome-Associated Membrane Glycoproteins Hydrogen-Ion Concentration Virus Internalization Lipids Cell biology 030104 developmental biology Infectious Diseases HEK293 Cells Membrane protein Acids |
| Zdroj: | Viruses, Vol 12, Iss 716, p 716 (2020) Viruses Volume 12 Issue 7 |
| ISSN: | 1999-4915 |
| Popis: | Lassa virus (LASV) is the causative agent of Lassa hemorrhagic fever, a lethal disease endemic to Western Africa. LASV entry is mediated by the viral envelope glycoprotein (GP), a class I membrane fusogen and the sole viral surface antigen. Previous studies have identified components of the LASV entry pathway, including several cellular receptors and the requirement of endosomal acidification for infection. Here, we first demonstrate that incubation at a physiological temperature and pH consistent with the late endosome is sufficient to render pseudovirions, bearing LASV GP, non-infectious. Antibody binding indicates that this loss of infectivity is due to a conformational change in GP. Finally, we developed a single-particle fluorescence assay to directly visualize individual pseudovirions undergoing LASV GP-mediated lipid mixing with a supported planar bilayer. We report that exposure to endosomal pH at a physiologic temperature is sufficient to trigger GP-mediated lipid mixing. Furthermore, while a cellular receptor is not necessary to trigger lipid mixing, the presence of lysosomal-associated membrane protein 1 (LAMP1) increases the kinetics of lipid mixing at an endosomal pH. Furthermore, we find that LAMP1 permits robust lipid mixing under less acidic conditions than in its absence. These findings clarify our understanding of LASV GP-mediated fusion and the role of LAMP1 binding. |
| Databáze: | OpenAIRE |
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