Genotypes and phenotypes of resistance in Ecuadorian Plasmodium falciparum

Autor:	Claudia A. Vera-Arias, Petra Rohrbach, Fabián E. Sáenz, Julio Valencia-Zamora, L. Enrique Castro, Gabriela Valenzuela
Rok vydání:	2019
Předmět:	0301 basic medicine lcsh:Arctic medicine. Tropical medicine Genotype lcsh:RC955-962 medicine.medical_treatment 030231 tropical medicine 030106 microbiology Plasmodium falciparum Resistance Genotypes Drug Resistance Protozoan Proteins Dihydroartemisinin Lumefantrine lcsh:Infectious and parasitic diseases 03 medical and health sciences chemistry.chemical_compound Antimalarials 0302 clinical medicine Parasitic Sensitivity Tests Chloroquine parasitic diseases medicine Humans lcsh:RC109-216 Artemether Malaria Falciparum Quinine biology Mefloquine Research medicine.disease biology.organism_classification Virology Malaria Phenotypes Infectious Diseases Phenotype chemistry Parasitology Ecuador medicine.drug
Zdroj:	Malaria Journal Malaria Journal, Vol 18, Iss 1, Pp 1-13 (2019)
ISSN:	1475-2875
Popis:	Background Malaria continues to be endemic in the coast and Amazon regions of Ecuador. Clarifying current Plasmodium falciparum resistance in the country will support malaria elimination efforts. In this study, Ecuadorian P. falciparum parasites were analysed to determine their drug resistance genotypes and phenotypes. Methods Molecular analyses were performed to search for mutations in known resistance markers (Pfcrt, Pfdhfr, Pfdhps, Pfmdr1, k13). Pfmdr1 copy number was determined by qPCR. PFMDR1 transporter activity was characterized in live parasites using live cell imaging in combination with the Fluo-4 transport assay. Chloroquine, quinine, lumefantrine, mefloquine, dihydroartemisinin, and artemether sensitivities were measured by in vitro assays. Results The majority of samples from this study presented the CVMNT genotype for Pfcrt (72–26), NEDF SDFD mutations in Pfmdr1 and wild type genotypes for Pfdhfr, Pfdhps and k13. The Ecuadorian P. falciparum strain ESM-2013 showed in vitro resistance to chloroquine, but sensitivity to quinine, lumefantrine, mefloquine, dihydroartemisinin and artemether. In addition, transport of the fluorochrome Fluo-4 from the cytosol into the digestive vacuole (DV) of the ESM-2013 strain was minimally detected in the DV. All analysed samples revealed one copy of Pfmdr1. Conclusion This study indicates that Ecuadorian parasites presented the genotype and phenotype for chloroquine resistance and were found to be sensitive to SP, artemether-lumefantrine, quinine, mefloquine, and dihydroartemisinin. The results suggest that the current malaria treatment employed in the country remains effective. This study clarifies the status of anti-malarial resistance in Ecuador and informs the P. falciparum elimination campaigns in the country.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d06536bfc85089139993ce36a21c63ef https://pubmed.ncbi.nlm.nih.gov/31822269 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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