Outer membrane vesicles isolated from two clinical Acinetobacter baumannii strains exhibit different toxicity and proteome characteristics
| Autor: | Ying Xian, Min Fan, Dan Xie, Xiao Jie Li, Zhi-Tao Li, Kou Xing Zhang, Lian Xu, Zhong Dao Wu, Ying Wang, Rui Ling Zhang, Xiaogang Bi |
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| Rok vydání: | 2014 |
| Předmět: |
Acinetobacter baumannii
Proteome Virulence Factors Virulence Microbiology Cell Line Mice Bacterial Proteins Tandem Mass Spectrometry Animals Humans Innate immune system biology Strain (chemistry) Cell Death Macrophages Secretory Vesicles Epithelial Cells biology.organism_classification GroEL Infectious Diseases Electrophoresis Polyacrylamide Gel Bacterial outer membrane Bacteria Acinetobacter Infections Chromatography Liquid |
| Zdroj: | Microbial pathogenesis. 81 |
| ISSN: | 1096-1208 |
| Popis: | Outer membrane vesicles (OMVs) are well-characterized virulence factors produced by Gram-negative bacteria. Here, we isolated two clinical Acinetobacter baumannii strains, the multidrug-resistant A. baumannii (MDRAb) A38 and non-MDRAb 5806. Strain A38 produced more abundant OMVs than strain 5806 when cultured to the early stationary phase. The results from cell proliferation assays and real-time PCR analyses indicated that A38 OMVs induced more powerful cytotoxicity and stronger innate immune responses compared with 5806 OMVs. Moreover, SDS-PAGE and LC-MS/MS analyses revealed that A38 OMVs contained more virulence factors, including Omp38, EpsA, Ptk, GroEL, hemagglutinin-like protein, and FilF. Taken together, the results of the present study suggest that MDRAb might produce abundant OMVs with more virulent factors facilitating the worse outcome, a finding that merits further study. |
| Databáze: | OpenAIRE |
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