Nanodroplet real-time PCR system with laser assisted heating
| Autor: | Gregory W. Faris, Christopher J. Green, Sanhita Dixit, Hanyoup Kim |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Materials science
Light Infrared Rays Microfluidics Nanotechnology Radiation Temperature measurement Polymerase Chain Reaction Article law.invention Heating Optics law TaqMan Miniaturization business.industry Reverse Transcriptase Polymerase Chain Reaction Lasers Far-infrared laser technology industry and agriculture Gene Amplification Temperature Substrate (chemistry) DNA Equipment Design Microfluidic Analytical Techniques Laser Atomic and Molecular Physics and Optics eye diseases Indium tin oxide Optoelectronics Thermodynamics business |
| Zdroj: | Optics express. 17(1) |
| ISSN: | 1094-4087 |
| Popis: | We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature measurement, and Taqman real-time readout in nanoliter droplets on a disposable plastic substrate. A selective heating scheme using an infrared laser appears ideal for driving PCR because it heats only the droplet, not the oil or plastic substrate, providing fast heating and completing the 40 cycles of PCR in 370 seconds. No microheaters or microfluidic circuitry were deposited on the substrate, and PCR was performed in one droplet without affecting neighboring droplets. The assay performance was quantitative and its amplification efficiency was comparable to that of a commercial instrument. |
| Databáze: | OpenAIRE |
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