Pectin Methacrylate (PEMA) and Gelatin-Based Hydrogels for Cell Delivery: Converting Waste Materials into Biomaterials
| Autor: | Alireza Dolatshahi-Pirouz, Gorka Orive, Mohammad-Ali Shahbazi, Ashish Thakur, Akhilesh K. Gaharwar, Mehdi Mehrali, Malgorzata Karolina Pierchala, Julio Alvin Vacacela Cordova, Firoz Babu Kadumudi, Mohammad Mehrali, Cristian Pablo Pennisi |
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| Rok vydání: | 2019 |
| Předmět: |
Pectin
muscle Biocompatible Materials 02 engineering and technology 01 natural sciences Gelatin Extracellular Matrix/chemistry bone PC12 Cells Extracellular matrix Human mesenchymal stem cells Tissue engineering General Materials Science neural Polysaccharide Biocompatible Materials/chemistry chemistry.chemical_classification pectin Cell Differentiation Hydrogels Polymer Cells Immobilized 021001 nanoscience & nanotechnology Extracellular Matrix Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Mesenchymal Stem Cell Transplantation/methods tissue engineering Self-healing hydrogels Muscle Methacrylates Pectins Methacrylates/chemistry 0210 nano-technology Gelatin/chemistry food.ingredient Materials science engineering.material 010402 general chemistry Methacrylate Mesenchymal Stem Cell Transplantation Cell Line gelatin human mesenchymal stem cells food Animals Humans Bone Neural Cells Immobilized/cytology Mesenchymal Stem Cells/cytology Mesenchymal Stem Cells Hydrogels/chemistry 22/4 OA procedure Pectins/chemistry 0104 chemical sciences Rats Hydrogel Chemical engineering chemistry Cell Differentiation/drug effects polysaccharide engineering Biopolymer hydrogel |
| Zdroj: | Mehrali, M, Thakur, A, Kadumudi, F B, Pierchala, M K, Cordova, J A V, Shahbazi, M-A, Mehrali, M, Pennisi, C P, Orive, G, Gaharwar, A K & Dolatshahi-Pirouz, A 2019, ' Pectin Methacrylate (PEMA) and Gelatin-Based Hydrogels for Cell Delivery : Converting Waste Materials into Biomaterials ', ACS Applied Materials & Interfaces, vol. 11, no. 13, pp. 12283-12297 . https://doi.org/10.1021/acsami.9b00154 Acs Applied Materials & Interfaces., 11, 12283-12297 Acs Applied Materials & Interfaces., 11, 13, pp. 12283-12297 ACS applied materials & interfaces, 11(13), 12283-12297. American Chemical Society |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.9b00154 |
| Popis: | The emergence of nontoxic, eco-friendly, and biocompatible polymers derived from natural sources has added a new and exciting dimension to the development of low-cost and scalable biomaterials for tissue engineering applications. Here, we have developed a mechanically strong and durable hydrogel composed of an eco-friendly biopolymer that exists within the cell walls of fruits and plants. Its trade name is pectin, and it bears many similarities with natural polysaccharides in the native extracellular matrix. Specifically, we have employed a new pathway to transform pectin into a ultraviolet (UV)-cross-linkable pectin methacrylate (PEMA) polymer. To endow this hydrogel matrix with cell differentiation and cell spreading properties, we have also incorporated thiolated gelatin into the system. Notably, we were able to fine-tune the compressive modulus of this hydrogel in the range ∼0.5 to ∼24 kPa: advantageously, our results demonstrated that the hydrogels can support growth and viability for a wide range of three-dimensionally (3D) encapsulated cells that include muscle progenitor (C2C12), neural progenitor (PC12), and human mesenchymal stem cells (hMSCs). Our results also indicate that PEMA-gelatin-encapsulated hMSCs can facilitate the formation of bonelike apatite after 5 weeks in culture. Finally, we have demonstrated that PEMA-gelatin can yield micropatterned cell-laden 3D constructs through UV light-assisted lithography. The simplicity, scalability, processability, tunability, bioactivity, and low-cost features of this new hydrogel system highlight its potential as a stem cell carrier that is capable of bridging the gap between clinic and laboratory. |
| Databáze: | OpenAIRE |
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