An important step in Listeria lipoprotein research
| Autor: | Francisco García-del Portillo, Pascale Cossart |
|---|---|
| Přispěvatelé: | Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Unite 604, Institut Naltional de la Santé et de la Recherche Médicale, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Virulence
MESH: Listeria monocytogenes medicine.disease_cause Genome MESH: Gram-Positive Bacteria Mice MESH: Gram-Negative Bacteria Aspartic Acid Endopeptidases Electrophoresis Gel Two-Dimensional Genetics 0303 health sciences Virulence biology 3T3 Cells MESH: Lipoproteins [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Proteome lipids (amino acids peptides and proteins) Peptide Termination Factors Lipoproteins In silico Gram-Positive Bacteria Signal peptidase II Microbiology 03 medical and health sciences Bacterial Proteins Listeria monocytogenes Transferases MESH: Transferases Gram-Negative Bacteria Operon medicine Animals Humans BACTERIE GRAM-NEGATIVE [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology Molecular Biology Gene 030304 developmental biology Molecular Biology of Pathogens BIOCHIMIE 030306 microbiology Computational Biology Gene Expression Regulation Bacterial biology.organism_classification BACTERIE GRAM-POSITIVE Microscopy Fluorescence Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mutation Listeria Guest Commentaries Caco-2 Cells Gene Deletion Lipoprotein |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2007, 189 (2), pp.294-297. ⟨10.1128/JB.01577-06⟩ Journal of Bacteriology, 2007, 189 (2), pp.294-297. ⟨10.1128/JB.01577-06⟩ |
| ISSN: | 0021-9193 1098-5530 |
| DOI: | 10.1128/JB.01577-06⟩ |
| Popis: | International audience; Over the last 10 years, DNA sequences of more than 600 bacterial species have been deposited in databases and are now available to search any gene, motif, or regulatory sequence of interest. Although genome data are instrumental in phylogenetic analysis and in silico design of metabolic and regulatory networks, only a very small fraction of the information has been experimentally validated. A striking example is lipoproteins predicted from genome sequences. Despite the predominance of this class of surface proteins in bacteria (up to 0.5 to 8% of the proteome), very few of these proteins have been identified as lipoproteins by biochemical methods (19). In this issue of the Journal of Bacteriology, Baumgärtner et al. (2) report a systematic analysis of lipoproteins of Listeria monocytogenes, a facultative gram-positive intracellular bacterial pathogen that causes severe infections (listeriosis) in both human and animals. These authors used an L. monocytogenes mutant defective in lipoprotein diacylglyceryl transferase (Lgt), an enzyme involved in lipoprotein processing. Three aspects of their study should be highlighted: (i) new findings concerning the roles of Lgt and lipoprotein-specific signal peptidase II (Lsp) during lipoprotein processing (22); (ii) the identification of 26 of the 68 lipoproteins predicted in the initial annotation of the L. monocytogenes strain EGD-e genome (7); and (iii) experimental evidence that a few of these lipoproteins are regulated by PrfA, the master virulence regulator of L. monocytogenes (8). Below, we discuss the significance of these findings separately. |
| Databáze: | OpenAIRE |
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