Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes
| Autor: | Daniel Surbek, Ruth Sager, Pierre Rollini, Reto Portmann, Andreina Schoeberlein, C. Bettina Portmann-Lanz, Stefan Mohr |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Cellular differentiation Placenta Mitosis Muscle Proteins Biology Placenta cord banking Neurotrophic factors Pregnancy Tubulin Neurosphere Internal medicine medicine Humans Progenitor cell 610 Medicine & health Neurons Obstetrics and Gynecology Cell Differentiation Mesenchymal Stem Cells 500 Science Immunohistochemistry Neural stem cell Cell biology Up-Regulation Oligodendroglia Endocrinology medicine.anatomical_structure 570 Life sciences biology Female Neuron Stem cell |
| DOI: | 10.7892/boris.225 |
| Popis: | Objective We aimed to induce neural stem (NSC) and progenitor cells (NPC) from human placental tissues. Study Design Placental stem cells from first-trimester placental chorionic villi and term chorion were isolated. Neural differentiation was initiated with plating on collagen, retinoic acid, and/or human brain-derived neurotrophic factor and epidermal and fibroblast growth factor. Differentiation into neurons, oligodendrocytes, and astrocytes was monitored by immunohistochemistry. Two-dimensional polyacrylamide gel electrophoresis, high-performance liquid chromatography, and tandem mass spectrometry were used to identify proteins involved in the differentiation. Results Differentiated cells were mostly immediately postmitotic with some more but not fully mature postmitotic neurons. Neurons had dopaminergic or serotonergic character. Some cells differentiated into predominantly immature oligodendrocytes. Upon differentiation, neuron-specific proteins were up-regulated, whereas placental proteins were reduced. Conclusion Stem cells derived from human placenta can be differentiated into neural progenitors. |
| Databáze: | OpenAIRE |
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