Author Correction: A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons
| Autor: | Thomas Perlmann, Markus Ringnér, Johan Holmberg, Konstantinos Toskas, Katarina Tiklova, Åsa K. Björklund, Erik Södersten, Vilma Rraklli |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Regulation of gene expression Multidisciplinary biology Science Dopaminergic General Physics and Astronomy General Chemistry Serotonergic General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 030104 developmental biology Histone nervous system Research council biology.protein lcsh:Q Psychology lcsh:Science Neuroscience |
| Zdroj: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018) |
| ISSN: | 2041-1723 |
| Popis: | The brain is composed of hundreds of different neuronal subtypes, which largely retain their identity throughout the lifespan of the organism. The mechanisms governing this stability are not fully understood, partly due to the diversity and limited size of clinically relevant neuronal populations, which constitute a technical challenge for analysis. Here, using a strategy that allows for ChIP-seq combined with RNA-seq in small neuronal populations in vivo, we present a comparative analysis of permissive and repressive histone modifications in adult midbrain dopaminergic neurons, raphe nuclei serotonergic neurons, and embryonic neural progenitors. Furthermore, we utilize the map generated by our analysis to show that the transcriptional response of midbrain dopaminergic neurons following 6-OHDA or methamphetamine injection is characterized by increased expression of genes with promoters dually marked by H3K4me3/H3K27me3. Our study provides an in vivo genome-wide analysis of permissive/repressive histone modifications coupled to gene expression in these rare neuronal subtypes. The limited size of some neuronal types and their entangled environment renders it difficult to study their transcription regulation. Here the authors present a comparative analysis of histone modifications and transcription in dopaminergic and serotonergic neurons and embryonic neural progenitors. |
| Databáze: | OpenAIRE |
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