MG53 promotes corneal wound healing and mitigates fibrotic remodeling in rodents
| Autor: | Tao Tan, T. M. Ayodele Adesanya, Jianjie Ma, Heather L. Chandler, Cornelia M. W. Peterson, Anne J. Gemensky-Metzler, Frank Yi, Xinyu Zhou, Qiang Wang, Rita F. Wehrman, Bingchuan Geng, Denis Kaili, Qiwei Jiang, Hua Zhu, Chunlin Yang |
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| Rok vydání: | 2019 |
| Předmět: |
genetic structures
Medicine (miscellaneous) Rodentia Article General Biochemistry Genetics and Molecular Biology Cornea Cell membrane 03 medical and health sciences 0302 clinical medicine Cell Movement Transforming Growth Factor beta In vivo Fibrosis medicine Animals Humans Regeneration lcsh:QH301-705.5 030304 developmental biology Mice Knockout Wound Healing 0303 health sciences business.industry Corneal Diseases Epithelium Corneal Membrane Proteins Fibroblasts medicine.disease Recombinant Proteins eye diseases Epithelium Rats 3. Good health Cell biology medicine.anatomical_structure lcsh:Biology (General) Membrane protein 030221 ophthalmology & optometry sense organs General Agricultural and Biological Sciences business human activities Homeostasis Corneal Injuries |
| Zdroj: | Communications Biology, Vol 2, Iss 1, Pp 1-10 (2019) Communications Biology |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-019-0316-7 |
| Popis: | The cornea plays an important role in transmitting light and providing protection to the eye, but is susceptible to injury and infection. Standard treatments for corneal wounds include topical lubricants, antibiotics, bandage contact lens, and surgery. However, these measures are often ineffective. Here we show that MG53, a protein with an essential role in cell membrane repair, contributes to the corneal injury-repair process. Native MG53 is present in the corneal epithelia, tear film, and aqueous humor, suggesting its potential function in corneal homeostasis. Knockout of MG53 in mice causes impaired healing and regenerative capacity following injury. Exogenous recombinant human MG53 (rhMG53) protein protects the corneal epithelia against mechanical injury and enhances healing by promoting migration of corneal fibroblasts. Using in vivo alkaline-induced injury to the rat cornea, we show that rhMG53 promotes re-epithelialization and reduces post-injury fibrosis and vascularization. Finally, we show that rhMG53 modulates TGF-β-mediated fibrotic remodeling associated with corneal injury. Overall, our data support the bi-functional role of MG53 in facilitating corneal healing and maintaining corneal transparency by reducing fibrosis and vascularization associated with corneal injuries. Heather Chandler, Tao Tan, Chunlin Yang et al. find that the cell membrane repair protein MG53 plays a key role in repairing cornea injury. Using mouse and rat models, they show that recombinant human MG53 protects the cornea against injury and enhances healing. |
| Databáze: | OpenAIRE |
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