A mitohormetic response to pro-oxidant exposure in the house mouse
| Autor: | Andreas N. Kavazis, Frances Humes, Wendy R. Hood, Yufeng Zhang, Gregory Almond |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.medical_specialty Time Factors Physiology Mitochondria Liver Mitochondrion Biology medicine.disease_cause Radiation Tolerance 010603 evolutionary biology 01 natural sciences Antioxidants Protein Carbonylation Lipid peroxidation Mice 03 medical and health sciences chemistry.chemical_compound Physiology (medical) Internal medicine medicine Animals Respiratory function Muscle Skeletal chemistry.chemical_classification Reactive oxygen species Pro-oxidant Mitochondria Muscle Oxidative Stress 030104 developmental biology Endocrinology Electron Transport Chain Complex Proteins chemistry Biochemistry Mitochondrial biogenesis Female Lipid Peroxidation House mice Reactive Oxygen Species Oxidative stress Research Article |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 314:R122-R134 |
| ISSN: | 1522-1490 0363-6119 |
| Popis: | Mitochondria are hypothesized to display a biphasic response to reactive oxygen species (ROS) exposure. In this study, we evaluated the time course changes in mitochondrial performance and oxidative stress in house mice following X-irradiation. Forty-eight mice were equally divided among six groups, including a nonirradiated control and five experimental groups that varied in time between X-ray exposure and euthanasia (1 h and 1, 4, 7, and 10 days after X-irradiation). We measured parameters associated with mitochondrial respiratory function and ROS emission from isolated liver and skeletal muscle mitochondria and levels of oxidative damage and antioxidants in liver, skeletal muscle, and heart tissues. Mitochondrial function dropped initially after X-irradiation but recovered quickly and was elevated 10 days after the exposure. Hydrogen peroxide production, lipid peroxidation, and protein carbonylation showed inverse U-shaped curves, with levels returning to control or lower than control, 10 days after X-irradiation. Enzymatic antioxidants and markers for mitochondrial biogenesis exhibited a tissue-specific response after irradiation. These data provide the first chronological description of the mitohormetic response after a mild dose of irradiation and highlight the protective response that cells display to ROS exposure. This study also provides valuable information and application for future mitochondrial and oxidative stress studies in numerous physiological settings. |
| Databáze: | OpenAIRE |
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