Effect of 21 Different Nitrogen Sources on Global Gene Expression in the Yeast Saccharomyces cerevisiae
| Autor: | Patrice Godard, Jacques van Helden, Antonio Urrestarazu, Stephan Vissers, Gianluca Bontempi, Kevin Kontos, Bruno André |
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| Přispěvatelé: | Laboratoire de Bioinformatique des Génomes et des Réseaux (BiGRe), Université libre de Bruxelles (ULB), Spinelli, Lionel |
| Rok vydání: | 2007 |
| Předmět: |
Protein Folding
Saccharomyces cerevisiae Proteins Transcription Genetic Nitrogen [SDV]Life Sciences [q-bio] Genes Fungal Saccharomyces cerevisiae Catabolite repression Regulon 03 medical and health sciences Gene Expression Regulation Fungal Urea Amino Acids Molecular Biology ComputingMilieux_MISCELLANEOUS Amino acid synthesis [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM] 030304 developmental biology 2. Zero hunger Regulation of gene expression chemistry.chemical_classification 0303 health sciences biology 030306 microbiology Gene Expression Profiling Fungal genetics Articles Cell Biology biology.organism_classification Orphan gene [SDV] Life Sciences [q-bio] Metabolic pathway Biochemistry chemistry [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2007, 27 (8), pp.3065--3086 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.01084-06 |
| Popis: | We compared the transcriptomes of Saccharomyces cerevisiae cells growing under steady-state conditions on 21 unique sources of nitrogen. We found 506 genes differentially regulated by nitrogen and estimated the activation degrees of all identified nitrogen-responding transcriptional controls according to the nitrogen source. One main group of nitrogenous compounds supports fast growth and a highly active nitrogen catabolite repression (NCR) control. Catabolism of these compounds typically yields carbon derivatives directly assimilable by a cell's metabolism. Another group of nitrogen compounds supports slower growth, is associated with excretion by cells of nonmetabolizable carbon compounds such as fusel oils, and is characterized by activation of the general control of amino acid biosynthesis (GAAC). Furthermore, NCR and GAAC appear interlinked, since expression of the GCN4 gene encoding the transcription factor that mediates GAAC is subject to NCR. We also observed that several transcriptional-regulation systems are active under a wider range of nitrogen supply conditions than anticipated. Other transcriptional-regulation systems acting on genes not involved in nitrogen metabolism, e.g., the pleiotropic-drug resistance and the unfolded-protein response systems, also respond to nitrogen. We have completed the lists of target genes of several nitrogen-sensitive regulons and have used sequence comparison tools to propose functions for about 20 orphan genes. Similar studies conducted for other nutrients should provide a more complete view of alternative metabolic pathways in yeast and contribute to the attribution of functions to many other orphan genes. |
| Databáze: | OpenAIRE |
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