Activation of human thymocytes by antibodies to the CD3/T-cell receptor complex: Triggering of different epitopes results in different signals
| Autor: | Henning Dr Sommermeyer, Reinhold E. Schmidt, Kurt Wonigeit, Reinhard Schwinzer, Hans-Jürgen Schlitt |
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| Rok vydání: | 1991 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte T cell receptor complex Thymus Gland/cytology CD3 Complex T-Lymphocytes CD3 Receptors Antigen T-Cell/physiology Immunology 610 Medizin Antigens CD/immunology CD2 Antigens Receptors Antigen T-Cell Thymus Gland In Vitro Techniques Biology Lymphocyte Activation Antigens CD3 CD2 molecule Epitope Antigens CD2 Calcium/physiology Epitopes Antigens CD Humans Receptors Immunologic Antibodies Monoclonal/immunology T-cell receptor Antibodies Monoclonal T lymphocyte T-Lymphocytes/immunology Flow Cytometry Receptors Immunologic/immunology Cell biology Thymocyte biology.protein Calcium Antigens Differentiation T-Lymphocyte/immunology Signal Transduction |
| Zdroj: | Cellular Immunology. 136:318-328 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(91)90355-f |
| Popis: | Monoclonal antibodies (mAb) against the CD3/T cell receptor (TcR) complex were analyzed for their ability to activate human thymocytes. In addition to mAb detecting epitopes on the CD3 complex (OKT3, BMA 030) the activation potential of recently developed mAb against common epitopes on the alpha/beta T-cell receptor (anti-TcR mAb: BMA 031, BMA 032) was evaluated. Several differences were observed between the two types of mAb: (a) Binding of the tested anti-CD3 mAb to thymocytes resulted in a rapid increase in the level of cytoplasmic free calcium ions [Ca2+]i, whereas no significant changes in [Ca2+]i were detected in thymocytes stimulated with BMA 031 or BMA 032. (b) Induction of effective proliferation induced by mAb OKT3 depended on exogenous IL-2 and in addition on the presence of accessory cells or phorbol-ester. Proliferation induced by BMA 031 only required exogenous IL-2. (c) OKT3 but not BMA 031 inhibited proliferation of thymocytes induced via the CD2 molecule. These studies indicate that anti-CD3 and anti-TcR mAb transduce different signals in thymocytes. Since the two types of mAb are directed to the same molecular complex the observed differences also support the idea that there are functionally different compartments in the CD3/TcR complex which may activate different signaling pathways. |
| Databáze: | OpenAIRE |
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