Simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in rat plasma by using high performance liquid chromatography-diode array detector
| Autor: | Nicola Costa, L. Di Marzio, Donatella Paolino, Marcello Locatelli, Massimo Fresta, Donato Cosco, A. Di Tullio, Christian Celia, Felisa Cilurzo, L. Malatesta |
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| Rok vydání: | 2018 |
| Předmět: |
Antimetabolites
Antineoplastic Clinical Biochemistry Pharmaceutical Science Irinotecan 01 natural sciences High-performance liquid chromatography Deoxycytidine Chemistry Techniques Analytical Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Pharmacokinetics Chromatography detector Drug Discovery Irinotecan Hydrochloride medicine Animals Spectroscopy Chromatography High Pressure Liquid Chromatography Chemistry 010401 analytical chemistry Plasma Antineoplastic Agents Phytogenic Gemcitabine 0104 chemical sciences Rats Standard curve 030220 oncology & carcinogenesis Injections Intravenous Camptothecin medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 159 |
| ISSN: | 1873-264X |
| Popis: | In this manuscript we aimed at the simultaneous separation and quantification of Gemcitabine and Irinotecan hydrochloride (injected both as single components and in combination) from Sprague Dawley rat plasma by using a validated method obtained through the use of a High Performance Liquid Chromatography (HPLC)-diode array detector (DAD). Gemcitabine and Irinotecan hydrochloride were detected and quantified using a Zorbax Extend C-18 column (250 mm × 4.6 mm; 5 μm particle size) in gradient elution mode. The chromatographic analyses were carried out in 15 min. The analytical mode was calibrated and validated in the concentration range from 0.1 to 18 μg/mL both for Gemcitabine and Irinotecan hydrochloride. Sprague Dawley rat plasma was used to perform the analysis. 3-methylxanthine was the internal standard. The weighted-matrix matched standard curves of Gemcitabine and Irinotecan hydrochloride showed a good linearity up to 18 μg/mL. Parallelism tests were also performed to evaluate whether the over-range samples could be analyzed after dilution without affecting the analytical performance. The intra- and inter-day precision (RSD%) values of Gemcitabine and Irinotecan hydrochloride were ≤7.14% and ≤11.5%, respectively. The intra- and inter-day trueness (Bias%) values were in the range from -11.5% to 1.70% for both drugs. The analytical mode performance was further tested after collecting Sprague Dawley rat plasma following a single-dose administration of chemotherapeutics or their association. The validated HPLC-DAD method allowed the simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in the rat plasma, besides the evaluation of the pharmacokinetic parameters and drug delivery. |
| Databáze: | OpenAIRE |
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