Growth characteristics of human bone marrow mesenchymal stromal cells at cultivation on synthetic polyelectrolyte nanofilms in vitro
Autor: | Dmitriy A. Reshetnikov, Maria G. Fomkina, N. O. Appazov, Lyudmila M. Mezhevikina, Saltanat Zh. Ibadullayeva, Evgeniy E. Fesenko |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Culture Mesenchymal stromal cells macromolecular substances 03 medical and health sciences 0302 clinical medicine Blood serum lcsh:Social sciences (General) Cytotoxicity lcsh:Science (General) Cell proliferation Multidisciplinary Chemistry Mesenchymal stem cell technology industry and agriculture Adhesion Polyelectrolytes Polyelectrolyte In vitro stomatognathic diseases 030104 developmental biology Biophysics Nanofilms Platelet lysate lcsh:H1-99 Polyallylamine hydrochloride 030217 neurology & neurosurgery Research Article lcsh:Q1-390 |
Zdroj: | Heliyon, Vol 7, Iss 3, Pp e06517-(2021) Heliyon |
ISSN: | 2405-8440 |
Popis: | This study examines the adhesive properties and cytotoxicity of polyelectrolyte nanofilms from polyethyleneimine (PEI), polyallylamine hydrochloride (PAH) and sodium polystyrene sulfonate (PSS) on human bone marrow mesenchymal stromal cells (h-MSCs) and mouse adipose tissue (m-MSC) in vitro. Films are formed on 24- and 96-well culture plates in the combinations: PEI, PAH, PEI-PSS, PEI-PSS-PAH, PEI-PSS-PEI. An analysis of the culture results show that direct contact of h-MSCs with the PEI surface promotes adhesion (93–95% of adhesive cells versus 40% in the control). On the PEI surface, h-MSCs are evenly distributed, form colonies and 80% monolayer after 72 h of culture, as in the control on culture plastic. On nanofilms from PAH and PEI-PSS-PAH, cells grow in the form of rosette-like colonies with long and thin processes similar to neurites. The cytotoxic properties of PSS were revealed in direct contact with h-MSCs (more than 40% of nonviable cells with damaged plasma membranes). On the PSS surface, cells lost their adhesiveness. To culture and stably grow the cell mass of h-MSCs, it is better to use monolayer nanofilms made of highly adhesive and non-toxic PEI polyelectrolyte, which can bind the growth factors of blood serum and platelet lysate, ensuring the growth of h-MSCs under in vitro deprivation conditions. Polyelectrolytes; Nanofilms; Mesenchymal stromal cells; Culture; Adhesion; Cell proliferation. |
Databáze: | OpenAIRE |
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