Human and Murine High Endothelial Venule Cells Phagocytose Apoptotic Leukocytes
| Autor: | Krista L. Hess, David S. Askew, Farzaneh Osati-Ashtiani, Kim Sue R S Tudor, Jacob D. Johnson, Joan M. Cook-Mills |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Phagocytosis
Lymphocyte Palatine Tonsil Cell High endothelial venules Apoptosis Bone Marrow Cells DNA Fragmentation Thymus Gland Biology Lymphatic System Mice Species Specificity Leukocytes medicine Lymph node stromal cell Animals Humans Lymphocytes Lymph node Organ Size Cell Biology medicine.anatomical_structure Cell culture Immunology Cancer research Corticosterone Spleen |
| Zdroj: | Experimental Cell Research. 236:404-411 |
| ISSN: | 0014-4827 |
| Popis: | Apoptotic cell death occurs during normal lymphocyte development and differentiation as well as following lymphocyte exposure to endogenous corticosteroids released during stress, malnutrition, and trauma. Recognition and engulfment of these apoptotic cells is important for the clearance of dying cells before they release potent inflammatory mediators into the vasculature or tissues. Phagocytosis of apoptotic cells is accomplished in part by macrophages. We report for the first time that apoptotic lymphocytes are also phagocytosed by high endothelial venule (HEV) cells. The murine HEV cell line mHEVa rapidly phagocytosed apoptotic lymphoid and myeloid cells with the greatest rate of phagocytosis occurring at 0-6 h. To confirm HEV cell interaction with apoptotic cells, we demonstrated that apoptotic human tonsil lymphocytes were phagocytosed by human tonsil HEV cells in primary cultures. Furthermore, we examined HEV cell phagocytosis in vivo. Mice were treated with a natural corticosterone (4-pregnene-11 beta,21-diol-3,20-dione) at levels detected during stress or malnutrition (93-180 micrograms serum cortisol/dl). At 4-12 h posttreatment, apoptotic lymphocytes were present inside vacuoles of HEV cells in axillary lymph node tissue sections, as determined by transmission electron microscopy. These data suggest that, in addition to macrophages, lymph node HEV cells also play a role in the removal of apoptotic lymphocytes. Moreover, since HEV cells are specialized endothelial cells that regulate lymphocyte migration into peripheral lymphoid tissues, they may provide an important checkpoint for clearance of apoptotic lymphocytes within the vasculature, as well as limiting entrance of nonfunctional lymphocytes into the lymph node. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect