Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats

Autor: Johan van der Stok, Huanan Wang, F. Cumhur Oner, Michelle T. Poldervaart, Sander C.G. Leeuwenburgh, Jacqueline Alblas, Wouter J.A. Dhert, Harrie Weinans
Přispěvatelé: Orthopedics and Sports Medicine
Rok vydání: 2013
Předmět:
Ceramics
Polymers
Cellular differentiation
Orthopedic Surgery
Bone Morphogenetic Protein 2
lcsh:Medicine
Biochemistry
Gelatin
law.invention
Engineering
Biopolymers
Subcutaneous Tissue
Glucuronic Acid
Biomimetics
Osteogenesis
law
Molecular Cell Biology
lcsh:Science
Microstructure
Multidisciplinary
Tissue Scaffolds
biology
Chemistry
Stem Cells
Goats
Hexuronic Acids
Porous Materials
Cell Differentiation
Animal Models
Anatomy
Controlled release
Microspheres
Cell biology
Smart Materials
Osteocalcin
Medicine
Female
Hydroxyapatites
Cellular Types
Material by Structure
Research Article
Biotechnology
Histology
Stromal cell
food.ingredient
Alginates
Materials Science
Biomedical Engineering
Mice
Nude
Bioengineering
Bone Marrow Cells
Bone morphogenetic protein 2
Material by Attribute
Biomaterials
Model Organisms
food
In vivo
Growth Factors
Animals
Humans
Rats
Wistar
Biology
3D bioprinting
Tissue Engineering
Multipotent Stem Cells
lcsh:R
Bioprinting
Proteins
Tissue engineering and pathology [NCMLS 3]
Delayed-Action Preparations
biology.protein
Composite Materials
Surgery
lcsh:Q
Stromal Cells
Zdroj: PLoS ONE, Vol 8, Iss 8, p e72610 (2013)
PLoS One, 8, 8
PLoS One (print), 8(8). Public Library of Science
PloS ONE, 8 (8), 2013
PLoS ONE
PLoS One, 8
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0072610
Popis: Contains fulltext : 119199.pdf (Publisher’s version ) (Open Access) The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the sustained release of bone morphogenetic protein 2 (BMP-2) influences osteogenicity of tissue engineered bioprinted constructs. BMP-2 loaded on gelatin microparticles (GMPs) was used as a sustained release system, which was dispersed in hydrogel-based constructs and compared to direct inclusion of BMP-2 in alginate or control GMPs. The constructs were supplemented with goat multipotent stromal cells (gMSCs) and biphasic calcium phosphate to study osteogenic differentiation and bone formation respectively. BMP-2 release kinetics and bioactivity showed continuous release for three weeks coinciding with osteogenicity. Osteogenic differentiation and bone formation of bioprinted GMP containing constructs were investigated after subcutaneous implantation in mice or rats. BMP-2 significantly increased bone formation, which was not influenced by the release timing. We showed that 3D printing of controlled release particles is feasible and that the released BMP-2 directs osteogenic differentiation in vitro and in vivo.
Databáze: OpenAIRE
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