Imaging regiospecific lipid turnover in mouse brain with desorption electrospray ionization mass spectrometry[S]
| Autor: | John C. Price, Bradley C. Naylor, Paul B. Farnsworth, Anna P. Zagieboylo, Mercede N. Erickson, Richard H. Carson, Charlotte R. Lewis, Kelvin W. Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Protein mass spectrometry Metabolite Analytical chemistry QD415-436 01 natural sciences Biochemistry Sample preparation in mass spectrometry diagnostic tools Mice 03 medical and health sciences chemistry.chemical_compound Endocrinology In vivo arachidonic acid Animals brain lipids Research Articles 010401 analytical chemistry Brain Stereoisomerism Cell Biology Metabolism Compartmentalization (fire protection) Lipid Metabolism molecular imaging Lipids 0104 chemical sciences 030104 developmental biology chemistry kinetics Biophysics diet and dietary lipids Molecular imaging Flux (metabolism) |
| Zdroj: | Journal of Lipid Research, Vol 58, Iss 9, Pp 1884-1892 (2017) |
| ISSN: | 0022-2275 |
| Popis: | Compartmentalization of metabolism into specific regions of the cell, tissue, and organ is critical to life for all organisms. Mass spectrometric imaging techniques have been valuable in identifying and quantifying concentrations of metabolites in specific locations of cells and tissues, but a true understanding of metabolism requires measurement of metabolite flux on a spatially resolved basis. Here, we utilize desorption ESI-MS (DESI-MS) to measure lipid turnover in the brains of mice. We show that anatomically distinct regions of the brain have distinct lipid turnover rates. These turnover measurements, in conjunction with relative concentration, will enable calculation of regiospecific synthesis rates for individual lipid species in vivo. Monitoring spatially dependent changes in metabolism has the potential to significantly facilitate research in many areas, such as brain development, cancer, and neurodegeneration. |
| Databáze: | OpenAIRE |
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