Hydrophobically modified polysaccharide-based on polysialic acid nanoparticles as carriers for anticancer drugs
| Autor: | Jong Ho Kim, Eun Hyang Jang, Min Ju Park, Man Kyu Shim, Hong Yeol Yoon, Kwangmeyung Kim, Bom Jung |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival Pharmaceutical Science Nanoparticle Antineoplastic Agents 02 engineering and technology 03 medical and health sciences Mice In vivo Cell Line Tumor medicine Animals Humans Doxorubicin Particle Size A549 cell Drug Carriers Polysialic acid Chemistry 021001 nanoscience & nanotechnology Xenograft Model Antitumor Assays In vitro Drug Liberation 030104 developmental biology Biochemistry Sialic Acids Nanoparticles Nanocarriers 0210 nano-technology Drug carrier Hydrophobic and Hydrophilic Interactions medicine.drug |
| Zdroj: | International journal of pharmaceutics. 520(1-2) |
| ISSN: | 1873-3476 |
| Popis: | This study presented the development of hydrophobically modified polysialic acid (HPSA) nanoparticles, a novel anticancer drug nanocarrier that increases therapeutic efficacy without causing nonspecific toxicity towards normal cells. HPSA nanoparticles were prepared by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling between N-deacetylated polysialic acid (PSA) and 5β-cholanic acid. The physicochemical characteristics of HPSA nanoparticles (zeta-potential, morphology and size) were measured, and in vitro cytotoxicity and cellular uptake of PSA and HPSA nanoparticles were tested in A549 cells. In vivo cancer targeting of HPSA nanoparticles was evaluated by labeling PSA and HPSA nanoparticles with Cy5.5, a near-infrared fluorescent dye, for imaging. HPSA nanoparticles showed improved cancer-targeting ability compared with PSA. Doxorubicin-loaded HPSA (DOX-HPSA) nanoparticles were prepared using a simple dialysis method. An analysis of the in vitro drug-release profile and drug-delivery behavior showed that DOX was effectively released from DOX-HPSA nanoparticles. In vivo cancer therapy with DOX-HPSA nanoparticles in mice showed antitumor effects that resembled those of free DOX. Moreover, DOX-HPSA nanoparticles had low toxicity toward other organs, reflecting their tumor-targeting property. Hence, HPSA nanoparticles are considered a potential nanocarrier for anticancer agents. |
| Databáze: | OpenAIRE |
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