Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa
| Autor: | Simon J. Waddell, Katherine A. Gould, Leticia Muraro Wildner |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Drug Tuberculosis media_common.quotation_subject Drug resistance Article Mycobacterium tuberculosis Efficacy 03 medical and health sciences Drug tolerance medicine Humans Tuberculosis Pulmonary media_common biology business.industry Gene Expression Profiling Sputum Gene Expression Regulation Bacterial medicine.disease Antimicrobial biology.organism_classification 030104 developmental biology Immunology medicine.symptom Transcriptome business |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493976362 |
| Popis: | The emergence of drug resistance threatens to destroy tuberculosis control programmes worldwide, with resistance to all first-line drugs and most second-line drugs detected. Drug tolerance (or phenotypic drug resistance) is also likely to be clinically relevant over the 6-month long standard treatment for drug-sensitive tuberculosis. Transcriptional profiling the response of Mycobacterium tuberculosis to antimicrobial drugs offers a novel interpretation of drug efficacy and mycobacterial drug-susceptibility that likely varies in dynamic microenvironments, such as the lung. This chapter describes the non-invasive sampling of tuberculous sputa and techniques for mRNA profiling M.tb bacilli during patient therapy to characterise real-world drug actions. |
| Databáze: | OpenAIRE |
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