Analysis of the Genome of Equine Herpesvirus Type 1: Arrangement of Cleavage Sites for Restriction Endonucleases EcoRI, BglII and BamHI
| Autor: | Andrew J. Davison, Whalley Jm, Robertson Gr |
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| Rok vydání: | 1981 |
| Předmět: |
Genes
Viral Equine herpesvirus 1 Population EcoRI HindIII Deoxyribonuclease EcoRI Restriction fragment Bacterial Proteins Virology Deoxyribonucleases Type II Site-Specific education Herpesviridae BglII Repetitive Sequences Nucleic Acid Genetics education.field_of_study Deoxyribonuclease BamHI biology Nucleic Acid Hybridization DNA Restriction Enzymes biology.organism_classification Molecular biology Molecular Weight Restriction enzyme DNA Viral biology.protein BamHI Herpesvirus 1 Equid |
| Zdroj: | Journal of General Virology. 57:307-323 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Summary The genome of an Australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases EcoRI, BglII and BamHI, and a physical map constructed. Terminal fragments were identified by exonuclease treatments, and linkage of fragments was deduced by a combination of single- and double-digest experiments and cross-blot hybridizations. The genome has a mol. wt. of 100 × 106 and is comprised of a short unique region bounded by repetitive sequences, which is present in both orientations in approximately equal amounts in the DNA population, and a long unique region existing in only one orientation. |
| Databáze: | OpenAIRE |
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