Detection of a bacteriophage gene encoding a Mu-like portal protein in Haemophilus parasuis reference strains and field isolates by nested polymerase chain reaction
| Autor: | Emilie S. Zehr, Louisa B. Tabatabai |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Haemophilus Infections
Genes Viral General Veterinary biology Swine Virulence biology.organism_classification Polymerase Chain Reaction Virology Molecular biology law.invention Bacteriophage Haemophilus parasuis law Haemophilus Animals Bacteriophages Gene Nested polymerase chain reaction Polymerase chain reaction |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 23:538-542 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | A nested polymerase chain reaction (nPCR) assay was developed to determine the presence of a gene encoding a bacteriophage Mu-like portal protein, gp29, in 15 reference strains and 31 field isolates of Haemophilus parasuis. Specific primers, based on the gene’s sequence, were utilized. A majority of the virulent reference strains and field isolates tested harbored the gene. The results suggest that the nPCR technique described in the current report could serve as a tool for epidemiological studies of H. parasuis. |
| Databáze: | OpenAIRE |
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