Label-Free Detection of Escherichia coli Based on Thermal Transport through Surface Imprinted Polymers
| Autor: | Olivier Deschaume, Kasper Eersels, Marloes Peeters, Erik Steen Redeker, Bart van Grinsven, Aleksandra Kordek, Hanne Diliën, Thomas J. Cleij, Sophie Ellermann, Carmen Bartic, Patrick Wagner, Onno Akkermans |
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| Přispěvatelé: | Maastricht Science Programme, RS: FSE MSP |
| Rok vydání: | 2016 |
| Předmět: |
Staphylococcus aureus
Materials science Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences heat-transfer method (HTM) Thermal transport Escherichia coli medicine Instrumentation Label free Fluid Flow and Transfer Processes chemistry.chemical_classification biology Process Chemistry and Technology Polymer 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences chemistry surface imprinted polymers Biophysics 0210 nano-technology point-of-care bacterial testing Bacteria |
| Zdroj: | ACS sensors, 1(9), 1140-1147. American Chemical Society |
| ISSN: | 2379-3694 |
| DOI: | 10.1021/acssensors.6b00435 |
| Popis: | This work focuses on the development of a label-free biomimetic sensor for the specific and selective detection of bacteria. The platform relies on the rebinding of bacteria to synthetic cell receptors, made by surface imprinting of polyurethane-coated aluminum chips. The heat-transfer resistance (R-th) of these so-called surface imprinted polymers (SIPs) was analyzed in time using the heat-transfer method (HTM). Rebinding of target bacteria to the synthetic receptor led to a measurable increase in thermal resistance at the solid liquid interface. Escherichia coli and Staphylococcus aureus were used as model organisms for several proof-of-principle experiments, demonstrating the potential of the proposed platform for point-of-care bacterial testing. The results of these experiments indicate that the sensor is able to selectively detect bacterial rebinding to the SIP surface, distinguishing between dead and living E. coli cells on one hand and between Gram-positive and Gram-negative bacteria on the other hand (E. coli and S. aureus). In addition, the sensor was capable of quantifying the number of bacteria in a given sample, enabling detection at relatively low concentrations (10(4) CFU mL(-1) range). As a first proof-of-application, the sensor was exposed to a mixed bacterial solution containing only a small amount (1%) of the target bacteria. The sample was able to detect this trace amount by using a simple gradual enrichment strategy. |
| Databáze: | OpenAIRE |
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