Characterization of Microbial Community in Cold-Chain Hairtail Fish by High-Throughput Sequencing Technology
| Autor: | Lingyan Mao, Xiaohu Luo, Jian Shen, Jingping Lu, Xiaorong Xu, Xing Jiali, Ruihang Zheng, Shufen Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Technology
Zoology Genus Pseudomonas Microbiology DNA sequencing 03 medical and health sciences Diversity index 0404 agricultural biotechnology Refrigeration RNA Ribosomal 16S Animals 030304 developmental biology 0303 health sciences Bacteria biology Microbiota High-Throughput Nucleotide Sequencing 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Microbial population biology Order Pseudomonadales Fish Species richness Food Science |
| Zdroj: | Journal of Food Protection. 84:1080-1087 |
| ISSN: | 0362-028X |
| Popis: | High-throughput DNA sequencing with the Illumina MiSeq platform was used to analyze the microbial communities in hairtail (Trichiurus haumela) muscle samples to study the diversity and dynamic changes in these communities during cold-chain circulation of these fish. The richness and diversity of the microbial community in hairtail muscle had a transient decline from 0 to 24 h and decreased after the first rise from 24 to 216 h. The diversity and richness of bacteria in cold-chain hairtail reached maximum at 168 h. The Shannon and Simpson diversity indices of the bacteria were 2.96 and 0.16, respectively, and their ACE and Chao1 richness indices were 254.84 and 155.10, respectively. The dominant bacteria belonged to phylum Proteobacteria, class Gammaproteobacteria, order Pseudomonadales, family Pseudomonadaceae, and genus Pseudomonas, and their relative abundances were 80.52, 72.11, 76.68, 23.25, and 53.50%, respectively. These results provide a basis for exploring how to maintain the freshness and predict the shelf life of hairtail. HIGHLIGHTS |
| Databáze: | OpenAIRE |
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