Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
| Autor: | George Seed, Penelope Flohr, Zafeiris Zafeiriou, Berni Ebbs, Mateus Crespo, Nikolas H. Stoecklein, Leon W.M.M. Terstappen, Lucy Hamilton, Claudia Bertan, Susana Miranda, Rita Pereira, Rui P L Neves, Diletta Bianchini, Alan Mackay, Ana Ferreira, Gemma Fowler, Ruth Riisnaes, Joanne Hunt, Maryou B. Lambros, Veronica Gil, Wei Yuan, Deirdre Moloney, Niven Mehra, Adam Sharp, Gunther Boysen, Daniel Nava Rodrigues, Suzanne Carreira, Jane Goodall, Rob Chandler, Ines Figueiredo, Kiki C. Andree, Pasquale Rescigno, Semini Sumanasuriya, Joost F. Swennenhuis, Johann S. de Bono, Mariane Sousa Fontes |
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| Přispěvatelé: | Medical Cell Biophysics |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Oncology Cancer Research medicine.medical_specialty Cell Count Cell Separation Somatic evolution in cancer Genetic Heterogeneity 03 medical and health sciences Prostate cancer 0302 clinical medicine Circulating tumor cell Internal medicine Biopsy Biomarkers Tumor medicine Humans Liquid biopsy In Situ Hybridization Fluorescence Comparative Genomic Hybridization medicine.diagnostic_test business.industry Liquid Biopsy 22/2 OA procedure High-Throughput Nucleotide Sequencing Prostatic Neoplasms Cancer Neoplastic Cells Circulating medicine.disease Cell Transformation Neoplastic 030104 developmental biology Apheresis Urological cancers Radboud Institute for Health Sciences [Radboudumc 15] 030220 oncology & carcinogenesis Blood Component Removal Cancer biomarkers Single-Cell Analysis business |
| Zdroj: | Clinical cancer research, 24(22), 5635-5644. American Association for Cancer Research Inc. Clinical Cancer Research, 24, 22, pp. 5635-5644 Clinical Cancer Research, 24, 5635-5644 |
| ISSN: | 1078-0432 |
| Popis: | Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR. |
| Databáze: | OpenAIRE |
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