A physiologically validated rat model of term birth asphyxia with seizure generation after, not during, brain hypoxia
| Autor: | Samu Kurki, Alexey S. Pospelov, Kai Kaila, Tommi Ala-Kurikka, Milla Summanen, Aleksander Alafuzoff, Juha Voipio |
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| Přispěvatelé: | Laboratory of Neurobiology, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, Helsinki Institute of Life Science HiLIFE, Joint Activities, Helsinki Institute of Life Science HiLIFE, Juha Voipio / Principal Investigator, Kai Kaila / Principal Investigator, Neuroscience Center |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Alkalosis POTENTIATION Encephalopathy neonatal seizures Neuroprotection ELECTROENCEPHALOGRAM ALKALOSIS EVENTS Asphyxia 03 medical and health sciences 0302 clinical medicine INJURY Animals Medicine Rats Wistar perinatal asphyxia RISK business.industry hypoxia 3112 Neurosciences Brain Reproducibility of Results Long-term potentiation hypercapnia Hypoxia (medical) medicine.disease EVOLUTION Rats Perinatal asphyxia Disease Models Animal 030104 developmental biology Animals Newborn Neurology Anesthesia Full‐length Original Research INTRACELLULAR PH neuroprotection Neurology (clinical) medicine.symptom business BURDEN Hypercapnia chemoreflex 030217 neurology & neurosurgery RESPONSES |
| Zdroj: | Epilepsia |
| Popis: | Objective Birth asphyxia (BA) is often associated with seizures that may exacerbate the ensuing hypoxic–ischemic encephalopathy. In rodent models of BA, exposure to hypoxia is used to evoke seizures, that commence already during the insult. This is in stark contrast to clinical BA, in which seizures are typically seen upon recovery. Here, we introduce a term‐equivalent rat model of BA, in which seizures are triggered after exposure to asphyxia. Methods Postnatal day 11–12 male rat pups were exposed to steady asphyxia (15 min; air containing 5% O2 + 20% CO2) or to intermittent asphyxia (30 min; three 5 + 5‐min cycles of 9% and 5% O2 at 20% CO2). Cortical activity and electrographic seizures were recorded in freely behaving animals. Simultaneous electrode measurements of intracortical pH, Po2, and local field potentials (LFPs) were made under urethane anesthesia. Results Both protocols decreased blood pH to |
| Databáze: | OpenAIRE |
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