A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein
| Autor: | Youngshim Lee, Woonwoo Lee, Youngdae Yoon, Hyo-Jin Kim, Yerin Kang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Green Fluorescent Proteins
Nanotechnology Biosensing Techniques Enhanced green fluorescent protein macromolecular substances split-protein systems lcsh:Chemical technology Biochemistry Article Analytical Chemistry Green fluorescent protein 03 medical and health sciences eGFP Escherichia coli lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Native structure 030304 developmental biology 0303 health sciences 030306 microbiology Chemistry technology industry and agriculture heavy metal biosensors Fluorescence Atomic and Molecular Physics and Optics Environmental Pollutants Biosensor Cadmium |
| Zdroj: | Sensors, Vol 19, Iss 8, p 1846 (2019) Sensors Volume 19 Issue 8 Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| Popis: | Microbial cell-based biosensors, which mostly rely on stress-responsive operons, have been widely developed to monitor environmental pollutants. Biosensors are usually more convenient and inexpensive than traditional instrumental analyses of environmental pollutants. However, the targets of biosensors are restricted by the limited number of genetic operon systems available. In this study, we demonstrated a novel strategy to overcome this limitation by engineering an enhanced green fluorescent protein (eGFP). It has been reported that combining two fragments of split-eGFP can form a native structure. Thus, we engineered new biosensors by inserting metal-binding loops (MBLs) between &beta strands 9 and 10 of the eGFP, which then undergoes conformational changes upon interaction between the MBLs and targets, thereby emitting fluorescence. The two designed MLBs based on our previous study were employed as linkers between two fragments of eGFP. As a result, an Escherichia coli biosensor exhibited a fluorescent signal only when interacting with cadmium ions, revealing the prospect of a new biosensor for cadmium detection. Although this study is a starting stage for further developing biosensors, we believe that the proposed strategy can serve as basis to develop new biosensors to target various environmental pollutants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |