CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression
| Autor: | Jacques Schrenzel, Christiane Wolz, Ludwig Stenz, Konstanze Pohl, Silvia Herbert, Tobias Geiger, Christiane Goerke, Patrice Francois, Frank Schlink |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Author's Correction
Threonine Staphylococcus aureus Virulence Factors Mutant Repressor Virulence Virulence Factors/biosynthesis Biology Microbiology Bacterial Proteins Gene Regulation Isoleucine Bacterial Proteins/biosynthesis/genetics/physiology Gene Psychological repression Molecular Biology Oligonucleotide Array Sequence Analysis Regulation of gene expression Genetics ddc:616 Gene Expression Profiling Trans-Activators/biosynthesis Wild type Gene Expression Regulation Bacterial Isoleucine/metabolism Staphylococcus aureus/metabolism/pathogenicity/physiology Repressor Proteins Mutation Trans-Activators Threonine/metabolism Repressor Proteins/genetics/physiology |
| Zdroj: | Journal of Bacteriology, Vol. 191, No 9 (2009) pp. 2953-63 |
| ISSN: | 1098-5530 |
| Popis: | The repressor CodY is reported to inhibit metabolic genes mainly involved in nitrogen metabolism. We analyzed codY mutants from three unrelated Staphylococcus aureus strains (Newman, UAMS-1, and RN1HG). The mutants grew more slowly than their parent strains in a chemically defined medium. However, only codY mutants were able to grow in medium lacking threonine. An excess of isoleucine resulted in growth inhibition in the wild type but not in the codY mutants, indicating that isoleucine plays a role in CodY-dependent repression. Prototypic CodY-repressed genes including the virulence regulator agr are repressed after up-shift with isoleucine. The CodY-dependent repression of agr is consistent with the concomitant influence of CodY on typical agr -regulated genes such as cap , spa , fnbA , and coa . However, some of these virulence genes (e.g., cap , fnbA , and spa ) were also regulated by CodY in an agr- negative background. Microarray analysis revealed that the large majority of CodY-repressed genes were involved in amino acid metabolism; CodY-activated genes were mainly involved in nucleotide metabolism or virulence. In summary, CodY in S. aureus not only acts as a repressor for genes involved in nitrogen metabolism but also contributes to virulence gene regulation by supporting as well as substituting for agr function. |
| Databáze: | OpenAIRE |
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