Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino
| Autor: | Jaime López M, Juan Carlos Roa S, Pablo Guzmán G, Carmen Gloria Ili G, José Manuel Ojeda F., Italo Capurro V, Patricia García M, Priscilla Brebi M, Eugenio Suárez P |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2010 |
| Předmět: |
Hpv genotypes
Gynecology medicine.medical_specialty Pathology Uterine cervical cancer Formalin fixed paraffin embedded biology Genotype business.industry Alpha papilloma virus General Medicine medicine.disease biology.organism_classification Squamous intraepithelial lesion Risk area Medicine Papillomaviridae business Viral genotype Uterine Cervical neoplasms |
| Zdroj: | Revista médica de Chile v.138 n.11 2010 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. Aim: To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. Material and Methods: Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. Results: HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. Conclusions: HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively. |
| Databáze: | OpenAIRE |
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