Activity of extracts and alkaloids of thai Alstonia species against human lung cancer cell lines
| Autor: | Peter J. Houghton, E. Eno-Amooquaye, P. J. Burke, Niwat Keawpradub |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lung Neoplasms
Magnetic Resonance Spectroscopy Villalstonine Stereochemistry Pharmaceutical Science Alstonia scholaris Pharmacognosy Adenocarcinoma Analytical Chemistry Alkaloids Drug Discovery Tumor Cells Cultured Humans Alstonia Pharmacology Plants Medicinal Apocynaceae biology Traditional medicine Indole alkaloid Plant Extracts Alkaloid Organic Chemistry biology.organism_classification Antineoplastic Agents Phytogenic Alstonia macrophylla Complementary and alternative medicine Molecular Medicine Carcinoma Large Cell |
| Zdroj: | Planta medica. 63(2) |
| ISSN: | 0032-0943 |
| Popis: | Methanol extracts of root barks of Alstonia macrophylla, A. glaucescens, and A. scholaris, collected from Thailand, have been assessed for cytotoxic activity against two human lung cancer cell lines, MOR-P (adenocarcinoma) and COR-L23 (large cell carcinoma), using the SRB assay. Significant cytotoxic activity was exhibited by the extract of A. macrophylla on both cell lines. Activity-directed fractionation led to the isolation of a novel indole alkaloid, O-methylmacralstonine, from the most active fraction of A. macrophylla along with four known alkaloids, talcarpine, villalstonine, pleiocarpamine, and macralstonine. Structure elucidation of the novel alkaloid was based on spectroscopic methods, especially 2D-NMR. The bisindole villalstonine was found to possess pronounced activity on both cell lines with an IC50 value less than 5 muM, but was about 10(3) times less potent than vinblastine sulphate. The monomeric alkaloid, talcarpine, was found to be inactive. Pleiocarpamine, O-methylmacralstonine and macralstonine were all considerably less active than villalstonine. |
| Databáze: | OpenAIRE |
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