Barrier function of reconstructed epidermis at the air-liquid interface: influence of dermal cells and extracellular components
Autor: | J. Wepierre, M. Aubery, M. Robert, I. Dusser, Marie-Sophie Noel-Hudson, M.-P. Muriel |
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Rok vydání: | 1997 |
Předmět: |
Keratinocytes
Male Cell Membrane Permeability Physiology Skin Absorption Dermatology Type IV collagen Laminin Skin Physiological Phenomena medicine Stratum corneum Humans Barrier function Cells Cultured Pharmacology biology Estradiol Water Cell Differentiation Membranes Artificial General Medicine Anatomy Fibroblasts Extracellular Matrix Fibronectin Chemically defined medium medicine.anatomical_structure Epidermal Cells Child Preschool Culture Media Conditioned biology.protein Biophysics Epidermis Keratinocyte Type I collagen Cell Division |
Zdroj: | Skin pharmacology : the official journal of the Skin Pharmacology Society. 10(5-6) |
ISSN: | 1011-0283 |
Popis: | To evaluate the epidermal barrier function of in vitro reconstructed epidermis, we measured the penetration of estradiol and water across human keratinocytes cultured in defined medium (DM), in the presence of proliferative fibroblasts (pF) or conditioned medium derived from pF, at the air-liquid interface on synthetic porous membrane, noncoated or coated with laminin, fibronectin, type I collagen or type IV collagen. Ultrastructural analysis showed a well-developed stratum corneum whatever the culture conditions. The permeability of reconstructed epidermis in DM on a noncoated porous membrane was 5- to 10-fold higher than human native epidermis, with both tracers. No significant change in barrier function was observed whatever the culture conditions. |
Databáze: | OpenAIRE |
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