Comparison of pp65 antigenemia, quantitative PCR and DNA hybrid capture for detection of cytomegalovirus in transplant recipients and AIDS patients

Autor: Mehdi Houimel, A. Slim, Leila Mhiri, Z. Arrouji, Belhassen Kaabi
Přispěvatelé: Laboratory of Virology, CHU Rouen, Normandie Université (NU)-Normandie Université (NU), Laboratoire d'Epidémiologie et d'Ecologie parasitaire, Institut Pasteur de Tunis, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire d'Immunopathologie, Vaccinologie et Génétique Moléculaire (LVGM)
Rok vydání: 2007
Předmět:
Tunisia
[SDV]Life Sciences [q-bio]
antigenemia
Congenital cytomegalovirus infection
hybrid capture
Biology
medicine.disease_cause
Polymerase Chain Reaction
Sensitivity and Specificity
Herpesviridae
law.invention
Viral Matrix Proteins
Immunocompromised Host
03 medical and health sciences
0302 clinical medicine
law
Betaherpesvirinae
Virology
medicine
Humans
030212 general & internal medicine
Antigens
Viral
cytomegalovirus
Polymerase chain reaction
Kidney transplantation
Bone Marrow Transplantation
0303 health sciences
AIDS-Related Opportunistic Infections
030306 microbiology
virus diseases
Viral Load
Phosphoproteins
medicine.disease
biology.organism_classification
Kidney Transplantation
3. Good health
qPCR
Cross-Sectional Studies
surgical procedures
operative
Real-time polymerase chain reaction
Cytomegalovirus Infections
DNA
Viral
Immunology
Viral disease
Viral load
Zdroj: Journal of Virological Methods
Journal of Virological Methods, Elsevier, 2007, 143 (1), pp.23-28. ⟨10.1016/j.jviromet.2007.01.033⟩
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2007.01.033
Popis: International audience; The cytomegalovirus (CMV) antigenemia assay has been used frequently for rapid diagnosis of CMV infection, and antigenemia threshold values are recommended for triggering preemptive therapy. Hybrid capture of CMV's DNA and quantitative polymerase chain reaction (qPCR) are increasingly being adopted for early detection of CMV. The performance of the antigenemia assay, qPCR in plasma and hybrid capture in leukocytes were compared in 110 immunocompromised patients (38 bone-marrow transplants, 50 renal transplants and 22 AIDS patients). The most sensitive test was hybrid capture for transplants, while antigenemia and the qPCR showed similar performance for patients with AIDS. QPCR and hybrid capture thresholds requiring antiviral therapy were calculated using a receiver-operating-characteristic curve for antigenemia values corresponding to 2 positive cells for bone-marrow transplants and to 10 positive cells for renal transplants and AIDS patients. These threshold values varied with the group of patients considered, with corresponding sensitivities higher than 86% and specificities higher than 76% for hybrid capture, and sensitivities higher than 61% and specificities higher than 75% for qPCR in plasma. Hybrid capture in leukocytes can substitute for antigenemia in the case of transplants, and qPCR in plasma can substitute for it in the case of AIDS patients. (C) 2007 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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